Pfam 17.0 (Saint Louis)
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PEPCK_ATP <-- --> PET

PEPcase


Accession number: PF00311
Phosphoenolpyruvate carboxylase

Description

Phosphoenolpyruvate carboxylase (PEPCase), an enzyme found in all multicellular plants, catalyses the formation of oxaloacetate from phosphoenolpyruvate (PEP) and a hydrocarbonate ion MEDLINE:1450389. This reaction is harnessed by C4 plants to capture and concentrate carbon dioxide into the photosynthetic bundle sheath cells. It also plays a key role in the nitrogen fixation pathway in legume root nodules: here it functions in concert with glutamine, glutamate and asparagine synthetases and aspartate amido transferase, to synthesise aspartate and asparagine, the major nitrogen transport compounds in various amine-transporting plant species MEDLINE:1421147.

PEPCase also plays an antipleurotic role in bacteria and plant cells, supplying oxaloacetate to the TCA cycle, which requires continuous input of C4 molecules in order to replenish the intermediates removed for amino acid biosynthesis MEDLINE:2779518. The C-terminus of the enzyme contains the active site that includes a conserved lysine residue, involved in substrate binding, and other conserved residues important for the catalytic mechanism MEDLINE:1508152.


Description text from InterPro entry IPR001449

Sequence information


Alignment

Seed (2)  Full (583)
Format:

Visualize domain structures

Seed (2)  Full (583)
display per page.

Species distribution

Tree depth:

Database References

PDB
1jqo1jqn1fiy1qb4
PRINTS PR00150
PROSITE PDOC00330
SCOP 1fiy (family)
INTERPRO IPR001449

HMMER build information

Pfam_ls [download HMM] Pfam_fs [download HMM]
Gathering cutoff 25.00 25.00 25.00 25.00
Trusted cutoff 31.00 31.00 27.50 27.50
Noise cutoff 18.90 18.90 19.60 24.00
Build method of HMM hmmbuild -F HMM_ls SEED
hmmcalibrate --seed 0 HMM_ls
hmmbuild -f -F HMM_fs SEED
hmmcalibrate --seed 0 HMM_fs

Pfam specific information

Author of entryFinn RD
Type definitionFamily
Alignment method of seed
Source of seed membersProsite


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