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DNA/RNA Preparation
Nucleic Acid Labeling
- Glass cDNA/Oligo Array: Probe Preparation Using Molecular Probes Ares Kit
(Download Adobe PDF)
- Reverse Transcription and aa-UTP Labeling of RNA
(Download Adobe PDF)
Protocol for making cDNA with aa-dUTP incorporation and labeling cDNA via chemical coupling of fluorescent dyes.
- Reverse Transcription and Labeling of Human RNA
(Download Adobe PDF)
Protocol for direct incorporation of fluorescent nucleotides into cDNA reverse transcribed from human mRNA.
- Modified Eberwine RNA Amplification
(Download Adobe PDF)
Protocol for amplifying RNA using a modified "ANTISENSE"(Eberwine) method.
- Labeling Genomic DNA Version A
(Download Adobe PDF)
Protocol for amplifying RNA using a modified "ANTISENSE"(Eberwine) method.
- Labeling Genomic DNA Version B
(Download Adobe PDF)
Protocols for labeling genomic DNA via direct incorporation or coupling for comparative genomic hybridizations.
- Amplification and Labeling of DNA
(Download Adobe PDF)
Protocol for amplifying and labeling DNA from ChIP or elsewhere.
Array Hybridization
- Array Hybridization
(Download Adobe PDF)
Protocol describing hybridization set up and probe application.
- Array Washing
(Download Adobe PDF)
PInstructions for washing microarrays after hybridization.
Supply list
- Supply Source Sheet
(Download Adobe PDF)
A comprehensive list of the supplies you need to run many of the protocols described above.
Credits:
These protocols were largely derived from the Cold Spring Harbor Laboratory Microarray Course manual. Contributors include: H.Bennett, J. DeRisi, V.Iyer, M. Diehn, A. Alizadeh, J.Lieb, and other members of the Brown and DeRisi labs.
You will need Adobe Acrobat Reader to view the documents.
Download Adobe Acrobat Reader Here.
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