Protocols
Cell culture and Transfection
RBL cell culture
Protocol for freezing down RBL cells
8-well transfection protocol using BTX cuvette porator
Mouse mast cell line cell culture (c57 cells)
Plating NIH-3T3 cells into 8-well coverslide
Plating and splitting NIH-3T3 fibroblasts
Transfecting NIH3T3 cells with Lipofectamine2000
Transfecting hippocampal neurons with Lipofectamine2000
Molecular Biology
Transformation
protocol using heat shock
Pouring agarose gel
Using
spectrophometer to quantitate DNA and RNA
Wondo's miniprep
PCR reactions and primer design
Tips for making new construct by PCR
Primer design for Gateway cloning system
Miscellaneous Protocols
Cytomix: 1) stock and 2) transfection buffer
Protocol for transfecting and fixing RBL cells using the BTX 8-well electroporator
Protocol for beta-hexosaminidase secretion assay in RBL cells
Protocol for grinding ESCAT coverslips
Accessing the lab construct database
Misc