DNA contanmination in RNA prep

Lu Z. H. zen.lu at ikp.unibe.ch
Fri Jul 7 09:31:44 EST 2000


I'm trying to look for a efficient method to remove the DNA
contanmination in my total RNA samples. I'm working with a Gram-positive
bacteria E. hirae and I'm trying to use the RNA for quantitative RT-PCR.

I've used both the Gaunidium thiocyanate method and the Qiagen's RNeasy
kit to extract my RNA. I then treated the RNA with DNase I. However, my
no RT control kept giving me positive signal in my PCR. Thank you!






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