II. BACTERIAL GROWTH AND MICROBIAL METABOLISM
1. Introduction to Photosynthesis
Learning Objectives for this Section
Autotrophs are organisms that are able to synthesize organic molecules (def) from inorganic materials. Photoautotrophs (def) absorb and convert light energy into the stored energy of chemical bonds in organic molecules through a process called photosynthesis.
Plants, algae, and bacteria known as cyanobacteria are known as oxygenic photoautotrophs because they synthesize organic molecules from inorganic materials, convert light energy into chemical energy, use water as an electron source, and generate oxygen as an end product of photosynthesis. Some bacteria, such as the green and purple bacteria, are known as anoxygenic phototrophs (def). Unlike the oxygenic plants, algae, and cyanobacteria, anoxygenic phototrophs do not use water as an electron source and, therefore, do not evolve oxygen during photosynthesis. The electrons come from compounds such as hydrogen gas, hydrogen sulfide, and reduced organic molecules. In this section on photosynthesis, we be concerned with the oxygenic phototrophs.
There are three major groups of photosynthetic bacteria: cyanobacteria, purple bacteria, and green bacteria.
In this section we will concentrate on oxygenic photosynthesis. Oxygenic photosynthesis is composed of two stages: the light-dependent reactions and the light-independent reactions. The light-dependent reactions convert light energy into chemical energy, producing ATP and NADPH. The light-independent reactions use the ATP and NADPH from the light-dependent reactions to reduce carbon dioxide and convert the energy to the chemical bond energy in carbohydrates such as glucose. Before we get to these photosynthetic reactions however, we need to understand a little about the electromagnetic spectrum and chloroplasts.
1. The Electromagnetic Spectrum
Visible light constitutes a very small portion of a spectrum of radiation known as the electromagnetic spectrum. All radiations in the electromagnetic spectrum travel in waves and different portions of the spectrum are catagorized by their wavelength. A wavelength is the distance from the peak of one wave to that of the next. At one end of the spectrum are television and radio waves with longer wavelengths and low energy. At the other end of the spectrum are gamma rays with a very short wavelength and a great deal of energy. Visible light is the range of wavelengths of the electromagnetic spectrum that humans can see, a mixture of wavelengths ranging from 380 nanometers (def) to 760 nanometers. It is this light that is used in photosynthesis.
Light and other types of radiation are composed of individual packets of energy called photons (def). The shorter the wavelength of the radiation, the greater the energy per photon. As will be seen shortly, when photons of visible light energy strike certain atoms of pigments during photosynthesis, that energy may push an electron from that atom to a higher energy level where it can be picked up by an electron acceptor in an electron transport chain (see Fig. 1). ATP can then be generated by chemiosmosis (def).
In eukaryotic cells, photosynthesis takes place in organelles called chloroplasts (see Fig. 2). Like mitochondria, chloroplasts are surrounded by an inner and an outer membrane. The inner membrane encloses a fluid-filled region called the stroma that contains enzymes for the light-independent reactions of photosynthesis. Infolding of this inner membrane forms interconnected stacks of disk-like sacs called thylakoids, often arranged in stacks called grana. The thylakoid membrane, which encloses a fluid-filled thylakoid interior space, contains chlorophyll and other photosynthetic pigments as well as electron transport chains. The light-dependent reactions of photosynthesis occur in the thylakoids. The outer membrane of the chloroplast encloses the intermembrane space between the inner and outer chloroplast membranes (see Fig. 2).
The thylakoid membranes contain several pigments capable of absorbing visible light. Chlorophyll is the primary pigment of photosynthesis. Chlorophyll absorbs light in the blue and red region of the visible light spectrum and reflects green light. There are two major types of chlorophyll, chlorophyll a that initiates the light-dependent reactions of photosynthesis, and chlorophyll b, an accessory pigment that also participates in photosynthesis. The thylakoid membranes also contain other accessory pigments. Carotenoids are pigments that absorb blue and green light and reflect yellow, orange, or red. Phycocyanins absorb green and yellow light and reflect blue or purple. These accessory pigments absorb light energy and transfer it to chlorophyll.
Photosynthetic prokaryotic cells do not possess chloroplasts. Instead, thylakoid membranes are usually arranged around the periphery of the bacterium as infoldings of the cytoplasmic membrane.
As mentioned above, photoautotrophs use sunlight as a source of energy and through the process of photosynthesis, reduce carbon dioxide to form carbohydrates such as glucose. The radient energy is converted to the chemical bond energy within glucose and other organic molecules.
The overall reaction for photosynthesis is as follows:
6 CO2 + 12 H2O in the presence of light and chlorophyll
C6H12O6 + 6 O2 + 6 H2O
Note that carbon dioxide (CO2) is reduced to produce glucose (C6H12O6 ) while water (H2O) is oxidized to produce oxygen (O2).
Photosynthesis is composed of two stages: the light-dependent reactions and the light independent reactions. We will now look at the role of each in the next two sections.
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