Prenatal Exposure to Diethylstilbestrol (DES)
in Males and Gender-Related Disorders:
Results from a 5-Year Study

by

Scott P. Kerlin, Ph.D.
DES Sons International Network
Vancouver, B.C., Canada
Skerlin2000@yahoo.com

August 2005

Paper prepared for the
International Behavioral Development Symposium 2005
Minot, North Dakota

Download PDF version of this paper

An earlier version of this paper was presented at
E.Hormone 2004, New Orleans
http://e.hormone.tulane.edu


Abstract

For many years, researchers and public health specialists have been assessing the human health impact of prenatal exposure to the estrogenic anti-miscarriage drug, diethylstilbestrol (commonly known as DES or "stilbestrol"). The scope of adverse effects in females exposed to DES (often called "DES daughters") has been more substantially documented than the effects in males ("DES sons"). This paper contributes three areas of important research on DES exposure in males: (1) an overview of published literature discussing the confirmed and suspected adverse effects of prenatal exposure in DES sons; (2) preliminary results from a 5-year online study of DES sons involving 500 individuals with confirmed (60% of sample) and suspected prenatal DES exposure; (3) documentation of the presence of gender identity disorders and male-to-female transsexualism reported by more than 100 participants in the study.

 

 

Introduction and Background


During the 1970s and 1980s an increased amount of public and scientific attention was paid to the health and medical problems of individuals whose mothers were prescribed diethylstilbestrol (DES). A potent synthetic nonsteroidal estrogen, DES was first developed in 1938 and initially became available in the U.S. for treating a range of gynecologic conditions in 1941 (Apfel and Fisher, 1984). A few years later its approval by the FDA was broadened to include treatment of pregnant women for the purpose of preventing miscarriages. Though its efficacy had long been questioned by some in the medical community (Bambigboye and Morris, 2003; Dieckmann, 1953), DES remained popular with doctors until discovery in the early 1970s of an apparent association between prenatal exposure to DES and a rare form of vaginal cancer in females whose mothers used DES (Heinonen, 1973; Herbst and Bern, 1981). Subsequent research confirmed the transplacental mechanism of DES transmission (Maydl, et al., 1983) and classified DES as a carcinogen and teratogen (Mittendorf, 1995) as well as a mutagen (Roy and Liehr, 1999; Stopper et al., 2005).

While DES usage with pregnant women was banned by the FDA in 1971, the drug continued to be used in several European countries into the early 1980s (Schrager and Potter, 2004). DES remained a popular option for treatment of advanced prostate cancer in aging males due to its ability to inhibit luteinizing hormone secretion by the pituitary and thus inhibit testosterone secretion (Scherr and Pitts, 2003; Whitesel, 2003), despite reports that adverse effects from this treatment could include feminization in males (B. C. Cancer Agency, 2005). Through the 1970s DES was also prescribed as an estrogen supplement for treatment of male-to-female transsexuals (Kwan, 1985; Ober, 1976).

It has been estimated that as many as four to five million American women were prescribed DES during pregnancy. Estimates of the numbers of "DES daughters" and "DES sons" born in the U.S. are between one million and three million of each (Edelman, 1986). Hundreds of thousands of DES sons and daughters were also born in Canada, Europe and Australia between the 1940s and 1980s. Efforts to determine exact numbers of prenatally exposed individuals, and the dosage and exposure patterns, particularly during the years of prime DES popularity, 1947-55 in the U.S., have been largely unsuccessful (Duke, et al., 2000; Heinonen, 1973). Because DES proved popular as a growth-stimulant in the cattle industry (Raun and Preston, 2002) for more than forty years (McLachlan, 2001), many consumers have also been exposed to unknown amounts of DES as it entered the food chain through beef consumption.

Following the FDA restrictions on DES prescriptions in the U.S. in 1971, researchers began to document a range of confirmed and suspected adverse effects of prenatal DES exposure in females and males (Edelman, 1986). Compared with the volume of published research on adverse effects in DES daughters, however, relatively few primary studies of DES sons have been published. The scope of known and suspected health effects among DES sons discussed in literature published since the 1950s includes (1) reproductive tract abnormalities; (2) infertility; (3) testicular cancer; (4) prostatic hyperplasia and cancer; (5) psychosexual effects; (6) psychiatric effects; and (7) effects on hemispheric laterality and spatial ability (Giusti et al., 1995; Pillard, et al., 1993; Reinisch and Sanders, 1992; Verdoux, 2004).

In 1959, a single case study of pseudohermaphrodism in a male with prenatal DES exposure was reported (Kaplan, 1959). Reports of urogenital abnormalities in DES sons first appeared in the medical literature during the 1970s (Andonian and Kessler, 1979; Bibbo et al., 1977; Cosgrove et al., 1977; Gill et al., 1979; Gill, et al., 1988; Henderson et al., 1976), consistent with results of experiments with prenatal DES exposure in male mice reported by McLachlan et al. (1975) and Newbold et al. (1987). These effects include epididymal (benign) cysts, hypoplastic or undescended testes (chryptorchidism), microphallus or underdeveloped penis, and hypospadias. Using data from DES sons participating in the DES cohort studies funded by the National Cancer Institute (NCI), researchers have examined possible links between prenatal DES exposure and greater risk of male infertility but have reached inconclusive findings (Wilcox et al., 1995). Although heightened testicular cancer risk has long been associated with prenatal DES exposure in males, researchers using the NCI cohorts to track cancer outcomes among DES sons claim to have measured no statistically significant increases in testicular or other forms of cancer (Strohsnitter, et al. 2001).

John McLachlan (2001), a pioneering DES researcher whose studies have assessed the effects of DES exposure in laboratory animals and mechanisms of DES toxicity for the past three decades, was among the first researchers to classify DES within a broader family of chemical compounds called "environmental estrogens", "xenoestrogens", or "endocrine disrupting chemicals" because of their common ability to mimic and interfere with normal hormonal processes associated with reproductive development. He has observed:

Developmental feminization at the structural or functional level is an emerging theme in species exposed, during embryonic or fetal life, to estrogenic compounds. Human experience as well as studies in experimental animals with the potent estrogen diethylstilbestrol provide informative models (2001).

The evolving research on endocrine disruptors has implicated DES in a variety of sexual differentiation disorders of the brain and body in males (Colburn et al, 1993; McLachlan et al., 2001; Sharpe, 2001; 2004; Sultan et al, 2001; Toppari et al., 1996), including testicular dysgenesis syndrome (Boisen, et al., 2001; Skakkebæk, Meyts, and Main, 2001). In 1993, Sharpe and Skakkebæk observed:

Treatment of several million pregnant women between 1945 and 1971 with a synthetic oestrogen diethylstilbestrol (DES) is now recognized to have led to substantial increases in the incidence of cryptorchidism and hypospadias and decreased semen volume and sperm counts in the sons of these women. DES exposure may also increase the incidence of testicular cancer and cryptorchidism. The similarity between these effects and the adverse changes in male reproductive development and function over the past 40-50 years raises the question of whether the adverse changes are attributable to altered exposure to oestrogens during fetal development. This possibility is not unlikely given the view that ‘humans now live in an environment that can be viewed as a virtual sea of oestrogens’ (Sharpe and Skakkebæk, 1993).

However, in a recent Danish meta-review of published epidemiological studies involving the association of prenatal indicators of estrogen exposure (including prenatal DES) and the deleterious impact on human male reproductive health such as reduced sperm counts, cryptorchidism, hypospadias and testicular cancer, Storgaard et al. (2005) reached this conclusion:

With the possible exception of testicular cancer there is no strong epidemiological evidence to indicate that prenatal exposures to estrogen are linked to disturbed development of the male reproductive organs. It is unlikely that phytoestrogens and several environmental xenoestrogens play a role unless exposures are extremely high (which is not expected), the dose–response relation is U-shaped or mixtures of xenoestrogens have synergistic actions. Low exposure levels for xenoestrogens may, however, operate by means of other toxicological mechanisms (Storgaard, et al., 2005).

It has been hypothesized that prenatal DES exposure may also have led to behavioral effects in humans (Meyer-Bahlburg and Erhardt, 1986; Meyer-Bahlburg, et al., 1995). Primary studies exploring possible behavioral and psychiatric effects of prenatal DES exposure in males first appeared in the literature during the 1970s. DES exposure has been associated with increased potential for major depressive disorders and other psychiatric effects in males (Katz, et al., 1987; Meyer-Bahlburg et al., 1985; Pillard et al., 1993; Saunders, 1988; Vessey et al., 1983). Recent discussions of potential psychiatric effects of prenatal DES exposure, including gender-related effects and schizophrenia, have been presented by Verdoux (2000; 2004) and Boog (2004). Verdoux summarizes the research on DES in the psychiatric literature this way:

Sparse findings suggest that exposure to xenoestrogens such as diethylstilbestrol may be a risk factor for psychiatric disorders, mediated by a possible deleterious impact of the substances on foetal neurodevelopment, but this hypothesis is speculative owing to the small number of studies and their methodological limitations (Verdoux, 2004).

Among the possible effects associated with prenatal DES exposure that have been discussed in the literature is impact on psychosexual development (Giusti et al., 1995). Research investigating possible psychosexual impact in human males was first published in the 1970s (Yalom, Green, and Fisk, 1973). Studies by Kester et al. (1980), Reinisch and Sanders (1984; 1992) and Reinisch, et al., (1991) attempted to assess various dimensions of "masculine" and "feminine" behavior and spatial ability among DES sons. In their meta-analysis of 19 studies on the behavioral effects of prenatal exposure to hormones administered for the treatment of at-risk human pregnancy (including the Yalom et al., 1973 and Kester et al., 1980 studies of DES-exposed males), Reinisch et al. (1991) concluded:

The data on prenatal exposure to synthetic estrogen derive primarily from subjects exposed to diethylstilbestrol (DES). DES-exposed male subjects appeared to be feminized and/or demasculinized, and there is some evidence that DES-exposed female subjects were masculinized.

A study of "psychosexual characteristics" (limited to questions regarding "handedness", "age at first sexual intercourse", "number of sexual partners", "percent having exclusively heterosexual partners", "percentage ever married") was conducted in 1994 with responses from DES sons and DES daughters participating in the National Cancer Institute’s long-term DES combined cohort studies (Titus-Ernstoff, et al. 2003). Although Udry (2003) critiques the Titus-Ernstoff study for not examining "gendered behaviors," no primary research investigating gender-related outcomes of DES-exposed males has been published since the Reinisch et al. review of 1991.

While it is not possible in this paper to review the extensive array of experimental animal research involving prenatal and neonatal DES exposure, two recent wildlife studies of the effects of DES on the reproductive function and behavior of male Japanese quail are notable. One study by Halldin et al. (2004) included DES in a primary assessment of the effects of estrogenic chemicals administered during the sexual differentiation phase in Japanese quail. They summarize:

We conclude that the Japanese quail is well suited as an animal model for studying various long-term effects after embryonic exposure to estrogenic compounds. Depressed sexual behavior is proved to be the most sensitive of the variables studied in males and we find this endpoint appropriate for studying effects of endocrine modulating chemicals in the adult quail following embryonic exposure.

A separate study of sexual behavior in male quail by Viglietti-Panzica et al. (2004) led to the conclusion:

The present data demonstrate that embryonic treatment with diethylstilbestrol induces a full sex reversal of behavioral phenotype as well as a significant decrease of vasotocin expression in the preoptic-limbic region in male Japanese quail.

These findings are consistent with those of Walker and Kurth (1993), who experimented with DES in laboratory mice and concluded that abnormal sexual differentiation of the fetal hypothalamus is the most common by-product of DES exposure.

Many questions remain as to how extensively the results of wildlife and animal behavioral studies involving DES can be extrapolated to measurable effects in humans (Vandenbergh, 2003; Zala and Penn, 2004). Questions with regard to the full impact of prenatal DES exposure on the genetic aspects of sexual differentiation have also been raised in recent years (Fielden, et al., 2002; Mericskay et al., 2005). These issues validate the importance of continued study and documentation of the developmental effects of DES exposure in animals as well as humans.

Researching DES Sons: An Internet Study

In July 1999, the U.S. National Cancer Institute, National Institute of Environmental Health Sciences, Office of Research on Women’s Health and the Centers for Disease Control jointly sponsored a two-day conference, "DES Research Update 1999: Current Knowledge, Future Directions" (NCI, 1999). The event brought together leading DES research scientists, public health specialists, and DES-exposed advocacy group representatives for an evaluation of what was known and what still needed further investigation in the realm of human health effects of DES exposure. Among the notable conclusions of this conference was that DES sons had been insufficiently studied, and that more studies were needed to document the full range of adverse health consequences in DES sons.

This present study was initially conceptualized as an Internet-based outreach campaign for locating DES sons from around the world and inspired by the need for more primary research involving DES sons. During the same month as the NCI’s DES conference, the DES Sons online network was launched at http://health.groups.yahoo.com/group/des-sons. Scott Kerlin, a DES son born in 1953, founded the network after extensive review of existing DES research and following a series of discussions with DES Action USA, the largest advocacy group representing DES-exposed individuals in the United States. In 2003, the network’s name was updated to "DES Sons International Network" in order to reflect the inclusion of DES sons located in Canada, Europe, and Australia. An extensive online reference library was also developed and maintained at http://health.groups.yahoo.com/group/des-sons/links.

The perceived advantages of utilizing the Internet for conducting this study included:

The network’s goals at the outset included (1) locating individual males who could confirm their prenatal DES exposure (i.e., confirmation that they are "DES sons"); (2) documenting the range of self-report indicators of lifetime health, medical, and behavioral concerns reported directly by DES sons; (3) promoting interpersonal support among DES sons; (4) expanding investigation of the confirmed and suspected adverse effects of prenatal DES exposure in males by surveying DES sons who had never participated in the NCI’s DES cohort studies.; (5) attempting to document the length of prenatal drug exposure including determination of the trimester of mother’s initial use of DES during pregnancy; and (6) assessing the level of public awareness about DES sons.

The revelation in the early 1970s of heightened cancer risk among DES daughters led to a public advocacy movement among DES daughters and their mothers for increased research on DES and women’s reproductive health concerns along with greater accountability among the drug companies (Seaman, 2003). However, DES sons have historically remained relatively isolated from one another and their health concerns have been largely unknown to the public. Among the activities of the DES Sons International Network was to document the most common patterns by which DES sons learned of their prenatal exposure. Researchers had long recognized that among DES daughters, the most common form of notification regarding DES exposure was from mothers (Apfel and Fischer, 1984; Seaman, 2003). Less has been known about communications and relationships between DES sons and their mothers although it is believed that lower percentages of DES sons than DES daughters have been informed of their exposure (NCI, 1999).

Sample Development

Upon launch of the DES Sons online network in 1999, announcements about the network were made through a variety of DES print and online outreach resources from DES Action USA, DES Action Canada, and DES Action Australia. Other announcements about the sons’ network and its web site were posted in male reproductive health resource networks where outreach was thought to provide greatest likelihood of reaching individual males with evidence of prenatal exposure.

Online requests for network memberships and listserv subscriptions became the mechanism by which, over time, the sample of DES sons was developed for the subsequent research study. Each request was carefully screened for (1) evidence or confirmation of prenatal DES exposure; (2) confirmation of birth between the late-1940s and early 1970s in all requests from individuals born in the U.S.; (3) confirmation that the subscriber was born as a male and thus qualified to be considered a "DES Son". There was no cost to participants who joined the network and all participation in subsequent interviews, surveys, and online discussions involved voluntary consent of the study participants. Members were asked to preserve the "closed" nature of all online discussions (i.e., access to list discussions was only for individuals who had become network members). In order to participate in the network’s discussion list, each membership applicant was asked by the researcher to provide a summary history of principal health, medical, and psychological issues that had occurred across the lifespan.

In accordance with recommended best practices in online health and medical research methodology, all health histories and online interview data gathered in this study were preserved confidentially offline and appropriate steps were followed to assure privacy (Duffy, 2002; Eysenbach, 2002; Sheehan and Hoy, 1999; Stone, 2003).

During 2003, the U.S. Centers for Disease Control and Prevention (CDC) held a year-long "DES Update" public education and outreach campaign for providing information to DES-exposed individuals (online at http://www.cdc.gov/DES/). The DES Sons International Network served as a participating partner and was the largest organization of DES sons to join the campaign. As a result, nearly 100 DES sons ultimately joined the online network in subsequent months.

Primary research on DES sons' health issues conducted through the network included (1) documenting each member’s self-report indicators of critical health, medical, and psychological events or issues across the lifespan; (2) periodic analysis and reporting of statistical data summaries of leading health concerns reported by DES-exposed members; (3) conducting several online surveys (open to network members only, and archived under the "polls" section of the DES sons network web site at http://health.groups.yahoo.com/group/des-sons/polls) on issues of reported greatest concern among network members; (4) follow-up interviews (open-ended) with individual DES sons, either online or by telephone when permission was granted for the researcher to make subsequent contact. Reports of research findings were posted annually to the DES Sons International Network in order to keep members aware of the range of primary health and medical concerns raised by network subscribers. A preliminary report summarizing what had been learned from research with DES sons during the first three years of the study, 1999-2002, was published by Kerlin and Beyer in 2003 (Kerlin and Beyer, 2003).

Study Statistics and Preliminary Findings

This paper’s Appendix presents an overview of statistics from initial analysis of data gathered during the primary study of DES sons discussed in this paper. The period of the full study spanned five years, from July 1999 to July 2004. What follows is a brief summary of the results that have been determined so far, based on feedback from more than 500 study participants. Data analysis will continue until 2006, when a full report will be released.

Sample Size
By July 2004, a sample of approximately 500 males with confirmed (60% of total) or "strongly suspected" DES exposure (40% of total) participated in the DES Sons International Network research and provided a summary of major health, medical, and psychological issues they had encountered across the lifespan. Among the 60% of participants who indicated they had confirmed their exposure, the majority of confirmations came from the mother’s verification of having been given DES at some time during the pregnancy. The total number of study participants who have confirmed their exposure through direct access to their mothers’ medical records continues to be investigated (see Appendix, Part I).

Nations of Origin
Approximately 85% of network members were born in the U.S., while 5% each indicated they were born in Canada, Europe (chiefly UK) or Australia.

Core Health Concerns of DES Sons
Based on preliminary analysis of critical health issues reported by individual DES sons in the network, the three topics most frequently listed among the sample of 500 individuals with confirmed or suspected prenatal DES exposure are (a) gender identity concerns (at least 150 reports); (b) psychological/mental health issues, especially depression and anxiety disorders (at least 100 reports); and (c) hormonal/endocrine health issues (at least 75 reports) (see Appendix, Part II).

Additional Reported Adverse Health Effects
Though identified less frequently in overall health reports provided by study participants, several participants listed histories of infertility, reproductive tract abnormalities (including reports of ambiguous or underdeveloped genitalia), epididymal cysts, cryptorchidism, hypospadias, gynecomastia, and erectile dysfunction. Statistics on the full extent of reporting of these concerns are still undergoing analysis.

Prevalence of Male-to-Female Transsexual, Transgender, and Intersex Individuals
More than 150 network members with "confirmed" or "strongly suspected" prenatal DES exposure identified as either "transsexual, pre- or post-operative," (90 members), "transgender" (48 members), "gender dysphoric" (17 members), or "intersex" (3 members). These statistics are taken from self-report terms provided by individual participants in their health histories (see Appendix, Part III).

Low Cancer Prevalence
Only 7 individuals with confirmed or "strongly suspected" prenatal DES exposure have reported experiencing some form of cancer. Most were testicular cancer survivors.

Discussion

In this study, more than 150 individuals with confirmed or suspected prenatal DES exposure reported moderate to severe feelings of gender dysphoria across the lifespan. For most, these feelings had apparently been present since early childhood. The prevalence of a significant number of self-identified male-to-female transsexuals and transgendered individuals as well as some individuals who identify as intersex, androgynous, gay or bisexual males has inspired fresh investigation of historic theories about a possible biological/endocrine basis for psychosexual development in humans, including sexual orientation, core gender identity, and sexual identity (Benjamin, 1973; Cohen-Kettenis and Gooren, 1999; Diamond, 1965, 1996; Michel et al, 2001; Swaab, 2004).

This study’s findings provide fresh evidence of psychiatric disturbances among individuals exposed to DES. It is hopeful that future research on human health effects of exposure to endocrine disrupting chemicals (i.e., assessing neurotoxicity) can include psychiatric disturbances such as major depression, anxiety disorders, eating disorders, and psychoses as potential endpoints for analysis of the long-term effects from prenatal exposure. Additional questions may be explored as to whether psychiatric conditions such as increased depression and/or anxiety disorders in DES sons have a foundation in primary endocrine system disorders.

Although the prevalence of endocrine system disorders among DES sons has not been discussed in any of the existing published epidemiological research on DES-exposed populations, both the Endocrine Society and the American Association of Clinical Endocrinologists (2002) have recognized prenatal DES exposure as a risk factor for endocrine disorders including hypogonadism. This study confirms that this issue needs further attention in future studies of DES sons.

While the rate of total cancer occurrence among members of the DES Sons International Network is uncertain, numerous efforts have been made to generate discussion about cancer risks and in particular, to encourage dialogue regarding testicular cancer experiences. Approximately seven members of the network between the study years of 1999 and 2004 indicated some past or present experience with testicular cancer. It appears that overall cancer outcomes among network members have been low, a finding consistent with research by Strohsnitter et al. (2001).


Implications

Although the scope of documented human health effects from prenatal exposure to various endocrine-disrupting chemicals continues to expand, the study of human behavioral effects is still in relative infancy (Ferguson, 2002; Swaab, 2004). This study’s findings may offer new insights for the emerging field of neurobehavioral teratology relative to understanding disturbances of gender identity and sexual identity development.

Undoubtedly the results of this study--particularly the findings with regard to the prevalence of gender-related concerns among a significant number of individuals with confirmed and/or suspected prenatal DES exposure--will come as a surprise for some. It is worth noting that investigations regarding the possible effects of prenatal DES exposure on sexual differentiation (brain and body), and sexual orientation have been undergoing discussion for quite some time (Baron-Cohen, 2004; Hines, 1998; Hines 1999; Meyer-Bahlburg et al., 1995; Toppari and Skakkebæk, 1998), though more emphasis in the published research has tended to be placed on possible effects in DES daughters than in DES sons.

While prior to this current study there have been no primary research studies of DES sons which have documented the prevalence of transsexualism or other gender identity disorders, there are publications in which prenatal DES exposure is listed among the potential factors associated with transsexualism or sexual differentiation disorders. For example, Michel, Mormont, and Legros (2001) in their psycho-endocrinological overview of transsexualism observe the following:

Gender identity disorders may be the consequence of an atypical hormonal environment such as congenital adrenal hyperplasia, resistance to androgens or even exogenous hormonal impregnation (the absorption of diethylstilboestrol treatment during pregnancy). In the majority of cases, these subjects do not develop towards transsexualism (2001, p. 366).

In the 6th edition of the widely-consulted Dictionary of Organic Compounds (1996) the DES entry appears on pages 2175-2176 and includes within its array of documented adverse effects, "causes male impotence and transsexual changes particularly in offspring exposed in utero." In the text, Human Embryology & Teratology, Second Edition (1996), O’Rahilly and Muller list DES among their directory of hormonal teratogens, stating, "Exposure of a female conceptus to DES, which can act as an estrogen, can lead to bisexuality. In a male conceptus, the secretion of testosterone can be suppressed, resulting in hypomasculinization." (O’Rahilly and Muller, 1996, p. 111).

The term "gender-bending chemicals" has become relatively popular with the news media in their latest reports on the toxic effects of endocrine disrupting chemicals such as phthalates on male reproductive development (Sample, 2005; Swan et al., 2005). Scarcely more than a decade ago, the concept was almost unheard of. Its introduction into early news stories describing documented and suspected but unconfirmed effects of endocrine disrupting chemicals (EDCs) no doubt provoked both amusement and angst in the public imagination (see "Gender-Bending Pollution", 1995). By the time the World Health Organization’s International Programme on Chemical Safety had released its "Global Assessment of the State-of-the-Science of Endocrine Disruptors" (IPCS, 2002), the story of DES had become part of the story of an entire group of environmentally-present toxic chemicals thought capable of creating a variety of reproductive abnormalities in humans as well as animal populations ("Alarm at Gender-Bending Chemicals", 2002). In that same year, Dutch researchers studying male and female children’s play behavior documented apparent "feminizing" effects in boys resulting from perinatal exposure to PCBs and dioxins (Vreugdenhil, et al., 2002). Undoubtedly, the issue of endocrine disruption and potential impact on gender identity and sexual development is an issue that merits wider investigation in the future (Johnson, 2004).

Historically, in the case of news stories about DES and its cancer-causing effects in DES daughters, many revelations first occurred in the 1970s (Berkson, 2000; Krimsky, 2000), but publicity regarding DES sons remained largely absent. And yet, there was no lack of recognition in the published medical literature that historically, at least some males prenatally exposed to DES were born with "structural and functional disorders of the reproductive tract" (Cosgrove, et al., 1977) or suffered psychiatric effects (Pillard et al, 1993).

If the results of this current study have pointed out anything significant, it is that we cannot relegate DES to the dustbin of "cancer-causing drugs no longer being used and therefore unworthy of continued investigation." And we cannot afford to limit the scope of our vigilance and public health information regarding long term effects of DES to cancer outcomes (Schrager and Potter, 2004).

Epilogue

A recent Cochrane Library Review of proposed medical protocols for evaluating future research identifying the relative risks and benefits (if any) of treating preterm infants with estrogens and progestins in order to prevent morbidity and mortality (Hunt et al., 2005) has recognized the history of adverse effects of prenatal DES exposure in sons and daughters. In discussing the history of adverse events associated with previous medical uses of estrogenic drugs for treatment of pregnancies, the authors observe:

Administration of sex steroids is not without risk. In the 1960's, women with high risk pregnancies were treated with diethylstilbestrol (DES). Epidemiological studies have since demonstrated strong associations between such therapy and abnormalities in the offspring of these pregnancies.

Perhaps most important relative to the findings presented in this current study of DES sons is the recommendation by Hunt et al. (2005) that future studies of preterm infants treated with estrogens and progestins need to carefully observe "evidence of any adverse events from hormone administration". Hunt et al. recognize two indicators of adverse events in this area:

 

Acknowledgments

I wish to thank Milton Diamond, Ph.D., University of Hawaii, John McLachlan, Ph.D., Center for Bioenvironmental Research at Tulane/Xavier Universities, Dana Beyer, M.D., (DES Sons International Network), Kathy Cochrane, and Christine Johnson for their helpful comments, suggestions and generous support.

 


References

"Alarm at Gender-Bending Chemicals." (2002, Aug. 12). Independent (London): 1.

American Association of Clinical Endocrinologists. (2002). Medical guidelines for clinical practice for the evaluation and treatment of hypogonadism in adult male patients--2002 update.
Online: http://www.aace.com/clin/guidelines/hypogonadism.pdf

Andonian, R. W., and R. Kessler. (1979). Transplacental exposure to diethylstilbestrol in men. Urology 13: 276.

Apfel, R. J., and Fisher, S. M. (1984). To do no harm: DES and the dilemmas of modern medicine. New Haven: Yale University Press.

B. C. Cancer Agency. (2005). Drug Database (professional): Diethylstilbestrol. Online: http://www.bccancer.bc.ca/HPI/DrugDatabase/DrugIndexPro/Diethylstilbestrol.htm

Baron-Cohen, S. (2004). Prenatal testosterone in mind. Cambridge, MA: MIT Press.

Bambigoye, A. A., and J. Morris (2003). Oestrogen supplementation, mainly diethylstilbestrol, for preventing miscarriages and other adverse pregnancy outcomes. The Cochrane Database of Systematic Reviews, Issue 3. Art. No.: CD004353. DOI: 10.1002/14651858.CD004353.

Benjamin, H. (1973). Transsexualism. American Journal of Nursing, 73: 457.

Berkson, L. (2000). Hormone Deception. New York: Contemporary Books.

Bibbo, M., W.B. Gill, F. Azizi, R. Blough, V. S. Fang, R. L. Rosenfield, G. F. B. Schumacher, K. Sleeper, M. G. Sonek, and G. L. Wied. (1977). Follow-up study of male and female offspring of DES-exposed mothers. Journal of Obstetrics and Gynecology (January) 49 (1): 1.

Boisen, K. A., K. M. Main, E. Rajpert-De Meyts, and N. E. Skakkebæk. (2001). Are male reproductive disorders a common entity?: The testicular dysgenesis syndrome. Annals of the New York Academy of Science 948: 90.

Boog, G. (2004). Obstetrical complications and subsequent schizophrenia in adolescent and young adult offspring: Is there a relationship? European Journal of Obstetrics & Gynecology and Reproductive Biology 114 (2): 130.

Cohen-Kettenis, P., and L. J. Gooren. (1999). Transsexualism: A review of etiology, diagnosis, and treatment. Journal of Psychosomatic Research, 46 (4): 315.

Colburn, T., F. S. vom Saal, and A. M. Soto. (1993). Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environmental Health Perspectives 101 (5): 378.

Cosgrove, M. D., B. Benton, and B. E. Henderson. (1977). Male genitourinary abnormalities and maternal diethylstilbestrol. Journal of Urology 177: 220.

Diamond, M. (1965). A critical evaluation of the ontogeny of human sexual behavior. Quarterly Review of Biology, 40: 147.

Diamond, M. (1996). Prenatal disposition and the clinical management of some pediatric conditions. Journal of Sex & Marital Therapy 22: 139.

Dictionary of Organic Compounds, 6th Edition. Diethylstilbestrol entry. London: Chapman & Hall, p. 2175.

Dieckmann, W. J., M. E. Davis, L. M. Rynkiewicz, and R. E. Pottinger. (1953). Does the administration of diethylstilbestrol during pregnancy have therapeutic value? American Journal of Obstetrics and Gynecology (November) 66: 1062.

Duffy, M. E. (2002). Methodological issues in Web-based research. Journal of Nursing Scholarship 34 (1): 83.

Duke, S. S., S. A. McGraw, and N. E. Avis. (2000). Exposure to diethylstilbestrol: Design of a multilevel community intervention. Health Promotion Practice 1 (2) 188.

Edelman, D. A. (1986). Diethylstilbestrol: New perspectives. Boston: MTP Press Ltd.

Eysenbach, G, and J. Wyatt. (2002). Using the Internet for surveys and health research. Journal of Medical Internet Research 4 (2); 13.

Ferguson, S. (2002). Effects on brain and behavior caused by developmental exposure to endocrine disrupters with estrogenic effects. Neurotoxicology and Teratology 24 (1): 1.

Fielden, M. R., R. G. Halgren, C. J. Fong, C. Staub, L. Johnson, K. Chou and T. R. Zacharewski. (2002). Gestational and lactational exposure of male mice to diethylstilbestrol causes long-term effects on the testis, sperm fertilizing ability in vitro, and testicular gene expression. Endocrinology 143 (8): 3044.

"Gender-bending pollution." (1995, July 25). Guardian: 6.

Gill, W. B., G. F. B. Schumacher, M. Bibbo, F.H. Straus, and H. W. Schoenberg. (1979). Association of diethylstilbestrol exposure in utero with cryptorchidism, testicular hypoplasia, and semen abnormalities. Journal of Urology, 122: 36.

Gill, W. B. (1988). Effects on human males of in-utero exposure to exogenous sex hormones. In T. Mori and H. Nagasawa (eds.), Toxicity of Hormones in Perinatal Life (Boca Raton: CRC Press), 161.

Giusti, R. M., K. Iwamoto, and E. E. Hatch. (1995). Diethylstilbestrol revisited: A review of the long-term health effects. Annals of Internal Medicine (May 15) 122 (10): 778.

Halldin, K., J. Axelsson, and B. Brunström (2004). Effects of endocrine modulators on sexual differentiation and reproductive function in male Jpanese quail. Brain Research Bulletin 65 (3): 211.

Heinonen, O. P. (1973). Diethylstilbestrol in pregnancy: Frequency of exposure and usage patterns. Cancer (March) 31: 573.

Henderson B. E., B. Benton, M. Cosgrove, J. Baptista, J. Aldrich, D. Townsend, W. Hart, and T. M. Mack. (1976). Urogenital tract abnormalities in sons of women treated with diethylstilbestrol. Pediatrics 58: 505.

Herbst, A. L., and Bern, H. A. (eds.) (1981). Developmental effects of diethylstilbestrol (DES) in pregnancy. N.Y.: Thieme-Stratton, Inc.

Hines, M. (1998). Abnormal sexual development and psychosexual issues. Bailliere’s Clinical Endocrinology and Metabolism 12: 173.

Hines, M. (1999). Gonadal hormones and sexual differentiation of human behavior: Effects on psychosexual and cognitive development. In A. Matsumoto (ed.), Sexual Differentiation of the Brain. N.Y.: CRC Press, p. 257.

Hunt R, P.G. Davis, and T. Inder. (2005). Replacement of estrogens and progestins to prevent morbidity and mortality in preterm infants (Cochrane Review). In: The Cochrane Library, Issue 2, 2005. Chichester, UK: John Wiley & Sons, Ltd.

International Programme on Chemical Safety, World Health Organization. (2002). Global assessment of the state-of-the-science of endocrine disruptors. Online: http://www.who.int/ipcs/publications/new_issues/endocrine_disruptors/en/ (last retrieved July 8, 2005).

Johnson, C. (2004). Transsexualism: An unacknowledged endpoint of developmental endocrine disruption? Unpublished Master’s thesis, Evergreen State University, Washington.

Kaplan, N. M. (1959) Male pseudohermaphrodism: Report of a case, with observations on pathogenesis. New England Journal of Medicine 261: 641.

Katz, D. L., F. R. Frankenburg, L. I. Benowitz, and J. M. Gilbert. (1987). Psychosis and prenatal exposure to diethylstilbestrol. Journal of Nervous and Mental Disease (May) 175 (3): 306.

Kerlin, S., and D. Beyer. (2003). The DES sons online discussion network. Transgender Tapestry 100.

Kester, P., R. Green, S. Finch, and K. Williams, (1980). Prenatal ‘female hormone’ administration and psychosexual development in human males. Psychoneuroendocrinology, 5: 269.

Krimsky, S. (2000). Hormonal chaos : The scientific and social origins of the environmental endocrine hypothesis. Baltimore: Johns Hopkins University Press.

Kwan, M., J. Vanmaasdam, and J.M. Davidson. (1985). Effects of estrogen treatment on male-to-female
transsexuals: Experimental and clinical observations. Archives of Sexual Behavior 14 (1):29.

Leary, F. J., L. J. Resseguie, L. T. Kurland, P. C. O’Brien, R. F. Emslander, and K. L. Noller. (1984). Males exposed in utero to diethylstilbestrol. Journal of the American Medical Association 252: 2984.

McLachlan, J. (2001). Environmental signaling: What embryos and evolution teach us about endocrine disrupting chemicals. Endocrine Reviews 22 (3): 319.

McLachlan, J. A., R. R. Newbold, and B. Bullock. (1975). Reproductive tract lesions in mice exposed prenatally to diethylstilbestrol. Science 190: 991.

McLachlan, J. A., R. R. Newbold, M. E. Burow, and S. Li. (2001). From malformations to molecular mechanisms in the male: Three decades of research on endocrine disrupters. APMIS 109 (4): 263.

Maydl, R., R. Newbold, M. Metzler, and J. A. McLachlan. (1983). Diethylstilbestrol metabolism by the fetal genital tract. Endocrinology 113: 146.

Mericskay, M., L. Carta, and D. Sassoon. (2005). Diethylstilbestrol exposure in utero: A paradigm for mechanisms leading to adult disease. Birth Defects Research Part A: Clinical and Molecular Teratology 73 (3): 133.

Meyer-Bahlburg, H. F. L., and A. A. Erhardt. (1986). Prenatal diethylstilbestrol exposure: Behavioral consequences in humans. Monograms in Neural Science 12: 90.

Meyer-Bahlburg, H. F. L., A. A. Erhardt, J. Endicott, N. Veridiano, E. D. Whitehead, and F. H. Vann. (1985). Depression in adults with a history of prenatal DES exposure. Psychopharmacology Bulletin 21 (3): 686.

Meyer-Bahlburg, H. F. L., A. A. Erhardt, L. Rosen, R. Gruen, V. F. H. Veridiano, and H. F. Neuwalder. (1995). Prenatal estrogens and the development of homosexual orientation. Developmental Psychology 31: 12.

Michel, A., C. Mormont, and J. J. Legros. (2001). A psycho-endocrinological overview of transsexualism. European Journal of Endocrinology 145: 365.

Mittendorf, R. (1995). Teratogen update: Carcinogenesis and teratogenesis associated with exposure to diethylstilbestrol (DES) in utero. Teratology 51 (6): 435.

Murray, P. J. (1997). Using virtual focus groups in qualitative research. Qualitative Health Research 7 (4): 542.

National Cancer Institute, NIH, National Institute of Environmental Health Sciences, NIH, Office of Research on Women's Health, NIH, & Centers for Disease Control and Prevention. (1999). DES Research Update 1999: Current Knowledge, Future Directions. Online: http://women.cancer.gov//DES/index.html (last retrieved: July 8, 2005)

Newbold, R. R., B. C. Bullock, and J. A. McLachlan. (1987). Mullerian remnants of male mice exposed prenatally to diethylstilbestrol. Teratogens, Carcinogens, Mutagens 7: 377.

Ober, W.B. (1976). Stilbestrol: A pathologist’s view. Pathology Annual 11: 227.

O’Rahilly, R., and F. Muller. (1996). Human embryology & teratology, 2nd edition. New York: Wiley-Liss.

Pillard, R. C., L.R. Rosen, H. F. L. Meyer-Bahlburg, J. D. Weinrich, J. F. Feldman, R. Gruen, and A. Ehrhardt. (1993). Psychopathology and social functioning in men prenatally exposed to diethylstilbestrol (DES). Psychosomatic Medicine 55: 485.

Raun, A. P., and R. L. Preston. (2002). History of diethylstilbestrol use in cattle. Unpublished paper, American Society for Animal Science, Online: http://www.asas.org/Bios/Raunhist.pdf

Reinisch, J. M., and S. A. Sanders (1984). Prenatal gonadal steroidal influences on gender-related behavior. Progress in Brain Research 61: 407.

Reinisch, J. M., and S. A. Sanders (1992). Effects of prenatal exposure to diethylstilboestrol (DES) on hemispheric laterality and spatial ability in human males. Hormones and Behavior 26 (1): 62.

Reinisch, J. M., Ziemba-Davis, M., and S. A. Sanders (1991). Hormonal contributions to sexually dimorphic behavioral development in humans. Psychoneuroendocrinology, 16, 213-278.

Roy, D. and J. Liehr. (1999). Estrogen, DNA damage and mutations. Mutations Research 424: 107.

Sample, I. (2005, June 3). Chemicals in plastics harming unborn boys. Guardian Weekly (UK): 6.

Saunders, E. J. (1988). Physical and psychological problems associated with exposure to diethylstilbestrol (DES). Hospital and Community Psychiatry 39(1): 73.

Scherr, D. S., and W. R. Pitts. (2003). The nonsteroidal effects of diethylstilbestrol: The rationale for androgen deprivation therapy without estrogen deprivation in the treatment of prostate cancer. Journal of Urology 170 (5): 1703.

Schettler, T., Solomon, G., Valenti, M., and A. Huddle. (1999). Generations at Risk: Reproductive Health and the Environment. Cambridge, MA: MIT Press.

Schrager, S., and B. Potter. (2004) Diethylstilbestrol exposure. American Family Physician 69 (10): 2395.

Seaman, B. (2003). The greatest experiment ever performed on women: Exploding the estrogen myth. New York: Hyperion.

Sharpe, R. M. (2001). Hormones and testis development and the possible adverse effects of environmental chemicals. Toxicology Letters 120: 221.

Sharpe, R. M. (2004). Environmental causes of testicular dysfunction. In S. J. Winters (ed.), Male hypogonadism: Basic, clinical, and therapeutic principles. Totowa, N.J.: Humana Press, 287.

Sharpe, R. M., and N. E. Skakkebæk. (1993) Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract? Lancet 341 (8857): 1392.

Sheehan, K, and M. Hoy. (1999). Using email to Internet users in the United States: Methodology and assessment. Journal of Computer-Mediated Communication 4 (3).

Skakkebæk, N. E., Meyts, E. Rajpert-De, and Main, K. M. (2001). Testicular dysgenesis syndrome: An increasingly common developmental disorder with environmental aspects. Human Reproduction, Vol. 5(5): 972.

Stone, J. (2003). Parry-Romberg syndrome: A global survey of 205 patients using the Internet. Neurology 61 (5): 674.

Stopper, H., E. Schmitt, and K. Kobras. (2005). Genotoxity of phytoestrogens. Mutation Research 574: 139.

Storgaard, L., J. P. Bonde, and J. Olsen. (2005) Male reproductive disorders in humans and prenatal indicators of estrogen exposure: A review of published epidemiological studies. Reproductive Toxicology (in press, August 2005).

Strohsnitter, W. C., K. L. Noller, R. N. Hoover, S. J. Robboy, J. R. Palmer, L. Titus-Ernstoff, R. H. Kaufman, E. Adam, A. L. Herbst, and E. E. Hatch. (2001). Cancer risk in men exposed in utero to diethylstilbestrol. Journal of the National Cancer Institute 93 (7): 545.

Sultan, C., P. Balaguer, B. Terouanne, V. Georget, F. Paris, C. Jeandel, S. Lumbroso, and J. Nicolas. (2001). Environmental xenoestrogens, antiandrogens and disorders of male sexual differentiation. Molecular and Cellular Endocrinology 178: 99.

Swaab, D. F. (2004). Sexual differentiation of the human brain: Relevance for gender identity, transsexualism and sexual orientation. Gynecological Endocrinology 19: 301.

Swaab, D. F., W. C. Chun, F. P. Kruijver, M. A. Hofman, and T. A. Ishunina. (2002). Sexual differentiation of the human hypothalamus. Advances in Experimental Medicine and Biology 511: 75.

Swan, S., K. M. Main, F. Liu, S. L. Stewart, R. L. Kruse, A. M. Calafat, C. S. Mao, J. B. Redmon, C. L. Ternand, S. Sullivan, J. L. Teague, and the Study for Future Families Research Team. (2005). Decrease in anogenital distance among male infants with prenatal phthalate exposure. Environmental Health Perspectives 113 (8): 1056.

Titus-Ernstoff, L., K. Perez, E. E. Hatch, R. Troisi, J. R. Palmer, P. Hartge, M. Hyer, R. Kaufman, A. Ervin, W. Strohsnitter, K. Noller, K. E. Pickett, and R. Hoover. (2003). Psychosexual characteristics of men and women exposed prenatally to diethylstilbestrol. Epidemiology (March) 14 (2): 155.

Toppari, J. and N. Skakkebæk. (1998). Sexual differentiation and environmental endocrine disruptors. Balliere’s Clinical Endocrinology and Metabolism 12: 143.

Toppari, J., J. C. Larsen, P. Christiansen, A. Giwercman, P. Grandjean, L. J. Guillette Jr., B. Jégou, T. K. Jensen, P. Jouannet, N. Keiding, H. Leffers, J. A. McLachlan, O. Meyer, J. Müller, E. Rajpert-De Meyts, T. Scheike, R. Sharpe, J. Sumpter, and N. E. Skakkebæk. (1996). Male reproductive health and environmental xenoestrogens. Environmental Health Perspectives 104 (S4): 741.

Udry, R. (2003). Putting prenatal effects of sex-dimorphic behavior in perspective: An absolutely complete theory. Epidemiology (March) 14 (2): 135.

Vandenbergh, J. (2003). Prenatal hormonal exposure and sexual variation. American Scientist 91 (3): 218.

Verdoux, H. (2000). Does prenatal exposure to diethylstilbestrol (DES) have psychiatric consequences? Annales Medico-Psychologiques 158 (2): 105.

Verdoux, H., and Behaud, B. (2004). Pharmaco-epidemiology: What do (and don’t) we Know About Utilisation and Impact of Psychotropic Medications in Real-Life Conditions? British Journal of Psychiatry.185: 93.

Vessey, M.P., D.V. Fairweather, B. Norman-Smith, and J. Buckley. (1983). A randomized double-blind controlled trial of the value of stilboestrol therapy in pregnancy: long-term follow-up of mothers and their offspring. British Journal of Obstetrics and Gynaecology 90(11): 1007.

Viglietti-Panzica, C., Montoncello, B., Mura, E., Pessatti, M., and Panzica, G. (2004). Organizational effects of diethylstilbestrol on brain vasotocin and sexual behavior in male quail. Brain Research Bulletin 65: 225.

Vreugdenhil, H. J. I., F. M. E. Slijper, P. G. H. Mulder, and N. Weisglas-Kuperus. (2002). Effects of perinatal exposure to PCBs and dioxins on play behavior in Dutch children at school age. Environmental Health Perspectives 110 (10): A593.

Walker, B. E., and L. A. Kurth. (1993). Pituitary tumors in mice exposed prenatally to diethylstilbestrol. Cancer Research 53: 1546.

Weiss, B. (1998). A toxic assessment perspective of the neurobehavioral toxicity of endocrine disruptors. Toxicology and Industrial Health 14 (1-2): 341.

Whitesel, J. A. (2003). The case for diethylstilbestrol. Journal of Urology 169 (1): 290.

Wilcox, A. J., D. D. Baird, C. R. Weinberg, P. P. Hornsby, and A. L. Herbst. (1995). Fertility in men exposed prenatally to diethylstilbestrol. New England Journal of Medicine (May 25) 332 (21): 1411.

Yalom, I. D., R. Green, and N. Fisk. (1973). Prenatal exposure to female hormones: Effect on psychosexual development in boys. Archives of General Psychiatry (April) 28: 554.

Zala, S. M., and Penn, D. J. (2004). Abnormal behaviors induced by chemical pollution: A review of the evidence and new challenges. Animal Behavior 68 (4): 649.


 

 

APPENDIX

DES Sons International Network 5-Year Summary Statistics

I. Statistics on DES Sons Participating in the DES Sons International Network Between 1999 and 2004

Online Study Dates: July 1999 to July 2004

    Totals
(a)
# of individuals requesting membership on DES Sons network listserv
600+
(b)
# of individuals (after initial screening) with confirmed or "suspected"
prenatal DES exposure who were allowed to join DES sons network
>500
(c)
% of (b) who confirmed prenatal DES exposure
60%
(d)
% of (b) with "suspected" but unconfirmed prenatal DES exposure
40%
(e)
% of (b) with direct access to mother’s medical records
Unknown
  1. In the five years since formation of the DES Sons network in July 1999, approximately 600 individuals requested information or support through e-mail follow-up requests and/or requests to join the network. This is over and above all information that is freely available for visitors to the Network’s web site (http://health.groups.yahoo.com/group/des-sons) which provides substantial information and resources on DES without subscription. Because the DES Sons International network does not maintain statistics on total Internet traffic to its web site, there is no accurate method to gauge how many other affected individuals may be utilizing this information.
  2. Of the 600 individuals who have sought further DES information, approximately 500 indicated at the time of my initial screening that they had either actual confirmation (from mother, or direct access to medical records) or strong suspicions (based on unconfirmed information from other family members) that they had been exposed to DES in utero. These 500 individuals with confirmed or suspected prenatal DES exposure were members of the network sometime between 1999 and 2004.

II. DES Sons Reported Health and Medical Concerns: Frequency of Reporting
     (Figures are based on a preliminary analysis of response data. A final report will be available in 2006.)

    Totals % of Total
(a)
Number of DES sons (confirmed exposure) participating in the
online study between 1999 and 2004
300
60%
(b)
Number of individual males with "suspected but unconfirmed"
prenatal DES exposure participating in study
200
40%
(c)
Numbers of DES sons (confirmed and suspected) reporting various health and medical concerns (percentages are of confirmed and suspected exposure):
 
   • Gender Dysphoria or Gender Identity Concerns
150+
30%
 
   • Major depression and/or anxiety disorders
100+
20%
 
   • Hypogonadism or other hormone-related disorders
75
15%
 
   • Infertility
30
6%
 
   • Testicular Cancer
7
1.4%
  1. Based on health history summaries received by the DES sons network between 1999 and 2004 from individuals with confirmed and suspected DES exposure, the three areas of greatest health concern among DES sons in the network appear to be (a) gender identity disturbances; (b) psychological/mental health issues including anxiety and depression; and (c) hormonal/endocrine health issues, especially hypogonadism. More than 150 members (all individuals who were born male) described histories of significant feelings of gender discomfort, and more than 90 identified as male-to-female transsexuals. More than 100 members described lifetime experiences with depression and/or anxiety disorders.
  2. Somewhat lower proportions of members indicated concerns regarding autoimmune disorders, infertility, reproductive tract abnormalities, ambiguous or underdeveloped genitalia, epididymal cysts, testicular cancer, and erectile dysfunction. Because not every individual member has necessarily disclosed the full range of health issues or medical concerns by which he or she has been affected, the relative significance of reported health concerns among DES sons in this research study is an approximation, based on preliminary textual analysis of information which has freely volunteered by network members.
  3. Cancer reports among DES sons were relatively rare (7 reported cases of testicular cancer).

III. Statistics of Prevalence of Transsexualism, Transgenderism, Gender Dysphoria, or Intersex Among "Confirmed" and "Suspected" DES Exposed Individuals (N=158)

  1. Among the population of DES sons joining the network who have discussed a history of gender identity concerns, personal stories and/or introductions have been received from more than 150 individuals with either confirmed or "strongly suspected" DES exposure.
  2. Responses were received from at least 93 individuals with confirmed prenatal DES exposure who self-identify as either transsexual (male-to-female), transgendered (male-to-female), "gender dysphoric," or intersex. The distribution of these 93 individuals is as follows:
Confirmed DES-Exposed and Gender-Related Issues
   
(N=93)
(1)
Confirmed Exposed and Transsexual:
54 individuals
(2)
Confirmed Exposed and Transgender:
26 individuals
(3)
Confirmed Exposed and Gender Dysphoric:
10 individuals
(4)
Confirmed Exposed and Intersex:
3 individuals
  1. There have been at least 65 individuals with "strongly suspected but not yet confirmed" exposure who indicated they are either either transsexual (male-to-female), transgendered (male-to-female), "gender dysphoric," or intersex. The distribution of these 65 individuals is as follows:
Strongly suspected, not confirmed DES Exposed and Gender-Related Issues
   
(N=65)
(1)
Suspected Exposure and Transsexual:
36 individuals
(2)
Suspected Exposure and Transgender:
22 individuals
(3)
Suspected Exposure and Gender Dysphoric:
7 individuals
(4)
Suspected Exposure and Intersex
None reported

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