Part A: Tissue Preparation

1. Intravascular Perfusion
   a.    Via Thoracotomy
   b.    Via Heart Puncture
   
2. LTP Physiology Protocol
3. Acquiring Tissue Region
   a.   Vibroslicer VT 1000S
   b.   Slice Dissection and Vibratoming in Agarose
   c.    Stoelting Tissue Chopper
   
4. Tissue Processing
   a.   Longhand
   b.   Microwave Enhanced Processing Schedules:
           i.   Standard Epon
          ii.   Epon/Spurrs
        iii.    Standard Epon with enhanced ribosomal 
          staining
   c.   Embedding of Tissue
          i.   Routine Embedding of Tissue
         ii.   Re-embedding Tissue
        iii.   resurfacing block
5. Tissue Preparation Safety Protocols
   a.    Neutralizing the Aldehydes
   b.    Disposing Osmium Tetroxide Contaminated Plastic Containers