Redissolving RNA Pellet
Gina Beck
glb7t at virginia.edu
Thu Sep 14 11:18:32 EST 2000
I am working with total RNA from Chondrocyte cells. I am isolating the
RNA using TRizol Reagent and I am having a problem with the last step of
redissolving the RNA pellet. These cells have been put in 1.2% alginate
and cultured for 5 days and then extracted out of the alginate using 55
uM of Na Citrate solution. Then I add Trizol and continue with the
isolation. Do you think there may be excess of proteoglycan material
that is making the pellet hard to redissolve? any suggestions?
Heather
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