C elegans Gene Knockout Protocols

One-Step Knockout with Chemical Mutagens:

• Reverse Genetics: Isolating Deletions in PCR Screens of Mutagenized Populations from the Barstead Lab.
• Gene Knockout Protocols from Michael Koelle's Lab
• Gene Knockout Protocol by TMP/UV Method (Ver. 1.3) (Hitoshi Inada and Ikue Mori)
• Chemical gene targeting from the Plasterk Lab
• Gene Knockout with Conventional Mutagens from Leon Avery.

• The C. elegans Gene Knockout Project
• Oligo design for screening deletion libraries from the Sanger Centre
• Reverse Genetics at NemaPharm

Tc1 insertion/deletion:

• Tc1 mediated target selected gene inactivation from the Plasterk Lab.

RNA interference

• the Mello Lab Home Page
• Fire Lab RNA interference documents (MacBinary format only)