Inverse PCR

[Malay]

Dear Netters:

Today my inverse PCR failed. I am trying to amplify a restriction fragment
from genomic DNA, where I know the sequence of half of the fragment. I am
trying to amply the other half. I ligated the fragment to form circles and
trying to amplify with primers from the known sequence. I am using Pfu for
amplification. Can anyone give me some suggestion/tips for inverse PCR using
Pfu?

Malay

Malay Kumar Basu
Centre for Cellular and Molecular Biology
Hyderabad 500007
I N D I A

Fax: (00-91)40-7171195
Phone: (00-91)40-7172241
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Santa's elves are just a bunch of subordinate Clauses.
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curiouser at ccmb.ap.nic.in




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