cDNA library screening with antibody

Tony Schountz tschount at mesastate.edu
Sun Jul 23 10:10:54 EST 2000


Can one use antibodies generated against whole cells to screen cDNA
expression libraries (lambda) for transmembrane proteins? Or does the TM
domain cause problems (e.g. ppt, screwed up folding, etc.)? I would
appreciate experienced opinions on this.

Thanks,

Tony








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