Prot. A Sepharose-Regeneration
Dr. Christian Klaus
klaus at gsf.de
Tue Jul 29 18:00:34 EST 1997
I am using Protein A Sepharose (Pharmacia) for immune precipitations. I
elute immune complexes after binding with laemmli buffer (SDS-PAGE
loading buffer).
Is there anyone who has experience, whether this very expensive material
can be regenerated and reused. I would be glad if some netter had a
protocol for me.
Thanks in advance
--
Dr. Christian Klaus
GSF-Forschungszentrum fuer Umwelt und Gesundheit
Institut fuer Strahlenbiologie
Postfach 1129
D-85758 Oberschleissheim
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