Prot. A Sepharose-Regeneration

Dr. Christian Klaus klaus at gsf.de
Tue Jul 29 18:00:34 EST 1997


I am using Protein A Sepharose (Pharmacia)  for immune precipitations. I
elute immune complexes after binding with laemmli buffer (SDS-PAGE
loading buffer). 
Is there anyone who has experience, whether this very expensive material
can be regenerated and  reused. I would be glad if some netter had a
protocol for me.

Thanks in advance
-- 
Dr. Christian Klaus
GSF-Forschungszentrum fuer Umwelt und Gesundheit
Institut fuer Strahlenbiologie
Postfach 1129
D-85758 Oberschleissheim



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