|
Molecular Biology Calculations
|
Calculations |
| OD260
to Concentration |
|
1 OD260
unit = |
50 µg/mL double stranded DNA
40 µg/mL RNA
33 µg/mL single stranded DNA |
| |
|
Double Stranded DNA |
ug DNA
1 mL |
x |
1 mL
103 µL |
x |
pmole
660 pg |
x |
106
pg
1 µg |
x |
1
N |
= |
pmole / µL |
pmole
µL |
x |
103 µL
mL |
x |
660 pg
pmole |
x |
µg
106 pg |
x |
N |
= |
µg / mL |
N = # bases
660 pg/µL = the average molecular weight of
a base pair |
| |
|
Oligos and Single Stranded DNA |
µg DNA
1 mL |
x |
1 mL
103 µL |
x |
pmole
330 pg |
x |
106
pg
1 µg |
x |
1
N |
= |
pmole / µL |
pmole
µL |
x |
103 µL
mL |
x |
330 pg
pmole |
x |
µg
106 pg |
x |
N |
= |
µg / mL |
N = # bases
330 pg/µL = the average molecular
weight of a base |
| |
|
Unit Conversion |
|
Prefix
|
Symbol
|
Factor
|
| kilo |
k |
103 |
| hecto |
h |
102 |
| deca |
da |
101 |
| deci |
d |
10-1 |
| centi |
c |
10-2 |
| milli |
m |
10-3 |
| micro |
µ |
10-6 |
| nano |
n |
10-9 |
| pico |
p |
10-12 |
| femto |
f |
10-15 |
|
