Contact  Intranet  Sitemap     EMBL Hamburg › Services › Protein Production and Characterisation › Protocols Macromolecular Crystallography Small Angle X-ray Scattering X-ray Absorption Spectroscopy Beamline User Guide Protein Production and Characterisation Protein Production Fermentation Characterisation Protocols Cloning Homologous Recombination Expression Purification Vectors and Strains Mass Spectroscopy HT-Crystallisation Technology Transfer Future Services: EMBL@PETRA3 Project Cloning Preparation of oligo solutions PCR experiments Digestion of insert DNA Digestion and dephosphorylation of vector DNA Ligation of DNA fragments with sticky ends Ligation of DNA fragments with blunt ends Preparation of chemically competent E. coli cells Transformation of plasmid DNA to chemically competent E. coli cells Last updated by: Administration, 31 May 2006 EMBL Web Support