Microarray Protocols

Microarray Protocols
Scientists at the OMRF Microarray Research Facility are available to address questions about cDNA production, labelling, and hybridizations. Many parameters have been tested and optimized for the particular equipment in our facility. We request that you cite our facility in publications if you find these protocols useful.

Protocols: Reagent and Supply List; Detailed Slide Blocking Protocol; Direct Cy-Labeling Protocol with Omniscript RT (Modified Labeling Protocol for Ventana Discovery System); Direct Cy-Labeling Protocol with EndoFree RT (Modified Labeling Protocol for Ventana Discovery System); Indirect Cy-Labeling Protocol (OMRF Modified Amersham CyScribe Labeling Protocol); Automated Hybridization Protocol (Ventana Discovery Optimized Protocol)
Frequently Asked Questions: How Much RNA Is Needed for Microarrays?; Which RNA Isolation Protocol Is Recommended?; Do you have other suggestions for working with RNA?; Do you print custom DNA libraries?
Validation: Quantitative Real-Time PCR (QRT PCR) Using Omniscript Reverse Transcriptase; Quantitative Real-Time PCR (QRT PCR) Using EndoFree Reverse Transcriptase

Send comments and updates to Dr. Bart Frank, Arthritis and Immunology Program, OMRF
Revised October 2002 by M. B. Frank, Ph.D.
Copyright 2002 by Mark Barton Frank, Ph.D.