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Overview | Description | Applications | Operations | Results | Publications | Images
Experiment/Payload OverviewThis study will investigate the susceptibility to fungal infection, progression of radiation-induced tumors and changes in immune function in sensitized Drosophila (fruit fly) lines.
Principal InvestigatorAmes Research Center, Moffett Field, CA
Sponsoring Space AgencyNational Aeronautics and Space Administration (NASA)
Supporting Organization:Exploration Systems Mission Directorate (ESMD)
Expeditions Assigned|13|
Previous ISS MissionsThis will be the first flight for the FIT experiment.
The Fungal Pathogenesis, Tumorigenesis, and Effects of Host Immunity in Space (FIT) investigation took place onboard the Space Shuttle during the STS-121/ULF1.1 mission. FIT addressed a series of human health risks
associated with space flight using Drosophila melanogaster (fruit flies) and the fungus Beauveria bassiana (B.bassiana fungus occurs in soils and can behave has a parasite on a number of insect species). Specifically, this research examined tumor progression and the compounding effect of radiation, and the progression of an immune response in the host in response to a pathogen in space.
The D. melanogaster were used to study the progression of oncogenic (cancerous) and benign melanotic tumors in sensitized mutant lines that show an increase in the incidence of tumor formation. Postflight samples were analyzed for changes in blood cell, hematopoietic organ (lymph gland) and fat body (liver) morphologies. The effect of radiation exposure was factored in to this study.
Additionally, fungal pathogens (B. bassiana) were exposed to radiation and the space environment; space flown samples were used for postflight infection of D. melanogaster hosts.
It is a known fact that space travel affects genetic activity of astronauts, but researchers can't yet predict which genes will be affected or precisely how gravity signals a gene to change. FIT is the first step in answering these questions.
Earth ApplicationsA better understanding of tumor progression and the effects of carcinogens is greatly relevant on Earth in efforts to cure cancer. Similarly, effects of pathogens on wild type and immunocompromised hosts are of great relevance to human immune diseases.
Inflight, the crew will conduct one session of the food tray change out operation. At the time of the food tray change out, the food tray exposed to the adult flies will carry eggs, embryo, and larvae. The procedure consists of transferring a new food tray into the fly cassette that carries the adult flies and then transferring the food tray with the larvae into a new unused fly cassette. The Platform Kit will be used to provide containment during this operation. Postflight, the specimens will be returned for processing by the PI team.
Operational ProtocolsDrosophila eggs will arrive on ISS in the larval form and hatch while on orbit. The flies will be housed in a special insect habitat that will be equipped with video cameras to allow researchers to monitor their behavior (e.g. courtship rituals, their running speed, how they fly), which are all clues to genetic activity. The flies will grow and breed, producing the foundation of approximately nine generations of flies.
The flies were returned to Earth on STS-121/ULF1.1; analysis of the flies is ongoing and results are expected in the near future. (Evans et al. 2009)