My research focus has been to initially identify novel factors, proteinacious or chemical, which contribute to the fitness of the plant pathogenic fungus, Botrytis cinerea. We are using a combination of tools including a bioinformatic analysis of the known genome sequence and LC-MS mass spectrometry to analyze the secretome of the organism upon exposure to various plant tissues. The LC-MS/MS analysis of various protein secretions is currently being performed in collaboration with Ron Orlando’s lab at the University of Georgia. The hypothesis that we are proposing is that the mechanism of pathogen attacks on host plants may be initially defined with the secretion of key proteins and chemicals that breach the plant cell wall as an initial barrier to the fungus’ attack on its host.
Howell, J. and Davis, M. R. (2005) "Plant Defense Mechanisms Against Fungal Pathogens: Polygalacturonase Inhibitor Proteins (PGIPs)". Can. J of Plant Physiol. 27(1): 5-15.
Davis, M.R. (2004) "Functional Genomics of Drought Stress Responses in Plants: A Review". Physiol. Mol. Biol. Plants, 10(1): 29-36.
Guo, S., Cichy, S.B., He, X., Yang, Q.,Ragland, M., Ghosh, A.K., Johnson, P.F., Unterman,T.G.. Insulin suppresses transactivation by CAAT/enhancer-binding proteins beta (C/EBPbeta). Signaling top300/CREB-binding protein by protein kinase B disrupts interaction with the major activation domain of C/EBPbeta.. J Biol Chem. 2001 276(11):8516-23.
Gay, P.A., Ragland, M., Breuning, G. and Soliman, K.M. Molecular characterization of tolerance to Aluminum toxicity in soybean. Phytopathology 1998 88:S31.Gay, P.A.,Ragland, M.Mosko,C. E., Tuzun, S. and Soliman,K.M. Molecular basis of tolerance to aluminum toxicity in soybean.Phytopathology1997 87:S32.
Ragland, M. and Soliman, K.M. “Sali5-4a and Sali3-2: Two Genes Induced by Aluminum in
Soybean Roots”.Plant Physiology, 114(1) PGR97-071, 1997.Ragland, M. and Theil, E.C. “Ferritin (mRNA, Protein) and Iron Concentrations During Soybean Nodule Development”. Plant Molec. Biol. 1993 (3):555-560.
Lescure, A.-M., Proudhon, D., Pesey, H., Ragland, M., Theil, E.C. and Briat, J.-F. “Ferritin Gene Transcription is Regulated by Iron in Soybean Cell Cultures”. Proc Natl Acad Sci U S A. 1991 88(18):8222-6.
Ragland, M., Briat, J.-F., Gagnon, J., Laulhere, J.-P., Massenet, O. and Theil, E.C. Evidence for
Conservation of Ferritin Sequences Among Plants and Animals and for a Transit Peptide in Soybean”. J Biol Chem. 1990. 265(30):18339-44.. 265(30):18339-44.