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Guidelines for
Selecting Route and Needle Size

General Guidelines on Injections & Technique

Guidelines for Maximal Injection Volumes
 

SPECIES

ROUTE

LOCATION

NEEDLE SIZE

Mouse

subcutaneous

dorsum, between scapula

25-26 ga

Mouse

*intramuscular

hindlimb, caudal thigh

26-27 ga

Mouse

intraperitoneal

lower left quadrant

25-27 ga

Mouse

**intravenous

lateral tail vein

27-28 ga

Mouse

intracerebral

cranium

27 ga, 1/8"

Rat

subcutaneous

dorsum, between scapula

25 ga

Rat

intramuscular

hindlimb, caudal thigh

26 ga

Rat

intraperitoneal

lower left quadrant

25-26 ga

Rat

intravenous

lateral tail vein, lateral saphenous vein

24-27 ga

Hamster

subcutaneous

dorsum, between scapula

25 ga

Hamster

intramuscular

hindlimb, caudal thigh

26 ga

Hamster

intraperitoneal

lower left quadrant

25-26 ga

Hamster

intravenous

lateral tarsal vein, cephalic vein, lingual vein

26 ga

Guinea pig

subcutaneous

dorsum, between scapula

25 ga

Guinea pig

intramuscular

hindlimb, caudal thigh, lumbar muscles

25 ga

Guinea pig

intraperitoneal

lower left quadrant

25 ga

Guinea pig

intravenous

lateral saphenous vein

26-27 ga

Guinea pig

intradermal

dorsum along flank

25 ga

Rabbit

subcutaneous

dorsum

22-25 ga

Rabbit

intramuscular

hindlimb, caudal thigh

22-25 ga

Rabbit

intraperitoneal

lower left quadrant

22 ga

Rabbit

intravenous

marginal ear vein

25 ga

Rabbit

intradermal

dorsum along flank

25 ga

Cats and Dogs

subcutaneous

dorsum, between scapula

22 ga

Cats and Dogs

intramuscular

hindlimb, caudal thigh

22-25 ga

Cats and Dogs

intravenous

cephalic, saphenous

22-25 ga

Swine

intramuscular

hindlimb, caudal thigh, lumbar muscles

20 ga

Swine

intravenous

marginal ear vein

22 ga

Sheep and Goats

subcutaneous

dorsum

20-22 ga

Sheep and Goats

intramuscular

hindlimb, caudal thigh, lumbar area

20-22 ga

Sheep and Goats

intravenous

cephalic vein

22 ga

Monkey

subcutaneous

dorsum

22-23 ga

Monkey

intramuscular

hindlimb, caudal thigh

22-23 ga

Monkey

intravenous

saphenous

22-23 ga

* Due to small muscle mass, this route is not recommended.

** To vasodilate the vessels and facilitate venipuncture, mice can be carefully warmed under heat lamps.

NOTE: Small rodents, including mice, rats, hamsters and guinea pigs should not be administered Freund's Complete Adjuvant intramuscularly.