# DILUTION OF AQUA-RESLIN® FOR THE BOTTLE BIOASSAY

According to the manufacturer’s label 1 gallon of AQUA-RESLIN® contains 1.67 pounds of permethrin and 1.67 pounds of piperonyl butoxide.

1 gallon = 3.785 liters

1 pound (lb.) = 453.6 grams.

1000 millligrams (mg)  = 1 gram

1000 micrograms = 1 milligram

1000 milliliters (ml) = 1 liter

1.67 lbs/gallon X 453.6 grams/lb. = 757.512 grams/gallon

757.5 grams/gallon divided by 3.785 liters/gallon = 200.135 grams/liter

1 liter of AQUA-RESLIN® contains 200.135 grams of permethrin

1 milliliter of AQUA-RESLIN® contains 0.2001 grams of permethrin

1 milliliter of AQUA-RESLIN® contains 200 milligrams of permethrin

TAKE one (1) milliliter of AQUA-RESLIN® and place it in a 100 ml volumetric flask.  Dilute it to 100 ml FINAL VOLUME with A.C.S. acetone [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.]  This is the STOCK solution.

The STOCK solution contains 0.002 grams of permethrin/ml

This is the same thing as 2 mg of permethrin per milliliter of STOCK solution.

TAKE 2.15 ml of STOCK solution and place it in a 100 ml volumetric flask.  Dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.  This is the TEST solution.

The TEST solution contains .043 mg of permethrin per milliliter.

The TEST solution contains 43 micrograms of permethrin per ml.

USE 1 ml of the TEST solution in each bottle of the bioassay.

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Complete instructions for the procedure for calculating time-mortality curves by the bottle bioassay are available at http://www.pherec.org/memoranda/procedure.html

For more information, CONTACT Dr. Jack Petersen at (850) 872-4184 extension 36 or email drjack3@hotmail.com

Thanks to Nancy Page and Jason Stuck, Pinellas County Mosquito Control District for assistance in the preparation of this PHEREC Technical Memorandum.

For a list of all PHEREC Technical Memoranda go to http://www.pherec.org/memoranda

####

20 May 2004 / jlp  15:50 p.m.// revised 25 May 2004