PHEREC
Technical Memorandum # 14

**DRAFT
protocol for the dilution of DUET for the Bottle Bioassay**

According
to the manufacturer’s label, DUET contains 2 active ingredients (AI) plus a
synergist, piperonyl butoxide. We are going to treat the
“off-the-shelf” product as if it contained only 1AI, Sumithrin^{®}

DUET
contains 0.37 pounds of Technical Sumithrin^{®}
/ gallon

CONVERT
pounds/gallon to grams/Liter

0.37
lbs/1 gal X 1 gal/3.78 L X 454 grams/pound = 44.44 grams / L

1
ml of DUET contains .044 grams Sumithrin^{®}

1.
Take 1 ml of DUET put it into a 100 ml volumetric flask. Add 99 ml A.C.S.
acetone. This is the STOCK solution.

.044
grams/ml X 1/100 = .00044 grams/ml

The
STOCK solution contains .00044 grams/ml

CONVERT
grams/ml to mg/ml

.00044grams/ml
X 1000 mg/ 1 gram = .44 mg/ml

.00044
grams/ml is equivalent to .44 mg/ml

2.
Take 5 ml of the STOCK and put it into a 100 ml volumetric flask. Add 95 ml A.C.S.acetone. This is the TEST solution.

5
ml X .44 mg/ml .022 mg/ml

The
TEST solution contains .022 mg/ml

CONVERT
mg to micrograms

.022
mg/ml is equivalent to 22 micrograms /ml

The
diagnostic dose of Sumithrin^{®
} is 22 micrograms/ml

Use
1 ml of the TEST solution in each 250 ml

Follow the standard protocol given at http://www.pherec.org/bottleassay

Edited June 1, 2007 jlp