DILUTION of ANVIL 10+10Ò for the BOTTLE BIOASSAY
DRAFT DOCUMENT FOR REVIEW
PHEREC Technical Memorandum # 1
During September 2001, three Florida Mosquito Control Programs requested assistance in evaluating ANVIL 10+10Ò as a mosquito adulticide. Workshops were conducted focusing on carrying out serial dilutions of ANVIL 10+10Ò to determine the diagnostic concentration to be applied to the interior surface of each 250 ml Wheaton bottle following the CDC protocol as modified by Dukes and Greer that is posted to the PHEREC Web site http://pherec.org/bottleassay/bottlebioassay.html
This brief technical memorandum reports the results of those workshops.
IMPORTANT MESSAGE: These technical notes are intended as a provisional report of on-going collaborative work. The conclusions contained in this memorandum may be modified in the future, based on additional laboratory or field experiences. Please go to the PHEREC Web site for updates http://pherec.org Direct inquiries and/or comments to: Dr. Jack Petersen, email@example.com
OBJECTIVE: Determine the diagnostic dose of ANVIL 10+10Ò for the bottle bioassay.
These preliminary results suggest that a dose of 22.17 ug of SUMITHRINÒ is a diagnostic dosage for the bottle bioassay.
This information supplements the final concentrations per bottle listed in Table 1 of the CDC protocol.
This information was obtained by testing Ochlerotatus taeniorhynchus field-collected in Pinellas and Monroe Counties. The conclusions stated in this technical memorandum may not apply to other mosquito species or to Oc. taeniorhynchus from other geographic locations.
Baseline data for susceptible Oc. taeniorhynchus are available by CLICKING HERE.
Abbreviations used in this document:
lbs = pounds
g = gram
mg = milligram
ug = microgram
l = liter
gal = gallon
1 gram (g) = 1000 milligrams (mg)
1 milligram (mg) = 1000 micrograms (ug)
Lot number: The research results reported here were obtained using ANVIL 10+10Ò lot number: 001820-6-1-A.
Three Florida Mosquito Control Districts participated in the work reported here: Citrus County MCD, Pinellas County MCD, and Keys MCD in Monroe County. Thank you to the directors and staff who made this work possible.
ANVIL 10+10Ò is a registered trademark of Clarke Mosquito Control Products, Inc., Roselle, Illinois 60172.
SUMITHRINÒ is a
registered trademark of Sumitomo Chemical Company, Ltd., Osaka, Japan
The chemical name of SUMITHRINÒ is d-phenothrin.
20 September 2001; revised 5 October 2001; revised 15 October 2001; revised 22 March 2002; revised 1 May 2002; revised 19 April 2004