DILUTION OF KONTROL 30-30 FOR THE BOTTLE BIOASSAY

DRAFT DOCUMENT FOR REVIEW

PHEREC Technical Memorandum # 2

According to the LABEL:

1 gallon of KONTROL 30-30 contains 2.5 lbs of permethrin

1 gallon = 3.785 liters
1 pound (lb.) = 453.6 grams

2.5 lbs X 453.6 g/lb = 1,134 grams a.i./gal.
1,134/3.785 = 299 grams/liter

1 liter of KONTROL 30-30 contains 299 grams of permethrin.
1 milliliter of  KONTROL 30-30 contains 0.299 grams of permethrin.
1 milliliter of  KONTROL 30-30 contains 299 milligrams of permethrin.

TAKE one (1) milliliter of KONTROL 30-30 CONCENTRATE and, using a volumetric flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.   [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.]  This is the STOCK solution.

The STOCK solution contains  2.99 milligrams/ml

TAKE 1.44 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.  This is the TEST solution.

2.99 milligrams/ml X 1.44ml= 4.3 milligrams of permethrin
4.3/100 = 0.043 milligrams/ml

This is the same thing as 43 micrograms/ml

The TEST solution contains 0.043 milligrams/ml permethrin
The TEST solution contains 43 micrograms/ml permethrin

USE 1 ml of the TEST solution in each bottle of the Bioassay.

For more information, CONTACT Dr. Jack Petersen
at (850) 872-4184 extension 36 or email drjack3@hotmail.com

Please check our Web site for updates on this protocol:
http://pherec.org