DILUTION OF PERMANONE 30-30 FOR THE BOTTLE BIOASSAY

DRAFT DOCUMENT FOR REVIEW

PHEREC Technical Memorandum # 5

Start with the label.  According to the label:

1 gallon of PERMANONE 30-30 contains 2.5 lbs of permethrin

1 gallon = 3.785 liters
1 pound (lb.) = 453.6 grams

2.5 lbs X 453.6 g/lb = 1,134 grams a.i./gal.
1,134/3.785 = 299 grams/liter

1 liter of PERMANONE 30-30 contains 299 grams of permethrin.
1 milliliter of  PERMANONE 30-30 contains 0.299 grams of permethrin.
1 milliliter of  PERMANONE 30-30 contains 299 milligrams of permethrin.

TAKE one (1) milliliter of PERMANONE 30-30  and, using a volumetric flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.   [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.]  This is the STOCK solution.

The STOCK solution contains  2.99 milligrams/ml

TAKE 1.44 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.  This is the TEST solution.

2.99 milligrams/ml X 1.44ml= 4.3 milligrams of permethrin
4.3/100 = 0.043 milligrams/ml

This is equivalent to 43 micrograms/ml

The TEST solution contains 0.043 milligrams/ml permethrin
The TEST solution contains 43 micrograms/ml permethrin

USE 1 ml of the TEST solution in each bottle of the bioassay according to the protocol by Dukes, et al.

The procedure reported here was field tested at Indian River Mosquito Control District during the week August 27-29, 2002.  I thank John Plate for assistance.

For more information, CONTACT Dr. Jack Petersen
at (850) 872-4184 extension 36 or email drjack3@hotmail.com

Please check our Web site for updates on this protocol:
http://pherec.org