**DILUTION OF BIOMIST 4+4 ULV FOR THE
BOTTLE BIOASSAY**

**DRAFT DOCUMENT FOR
REVIEW**

PHEREC Technical Memorandum # 3

The following procedure yields permethrin at a dosage appropriate for testing insecticide resistance by means of the bottle bioassay as determined by repeated test runs at PHEREC.

- Start with the label.
- According to the label one gallon of BIOMIST 4+4 ULV contains 0.3 pounds of permethrin.
- Convert pounds/gallon to grams/liter.
- 0.3 pounds/ 1 gallon X 453.6 grams/ 1 pound X 1 gallon/ 3.785 liters = 35.952 grams / liter
- Convert grams / liter to milligrams/milliliter
- 35.952 grams / liter X 1 gram / 1000 milligrams X 1000 milliliters / 1 liter = 35.952 milligrams / milliliter
- Dilute 1 milliliter of BIOMIST 4 + 4 ULV in 100 milliliters A.C.S. acetone.
- 35.952 milligrams / milliliter X 1 ml / 100 ml = 0.359 mg / ml
- This is the STOCK solution.

The STOCK solution can be stored in a light proof sealed container until needed.

The TEST solution is prepared as follows:

- Pipette 12 ml of the STOCK solution into a 100 ml volumetric flask.
- Fill the volumetric flask to the 100 ml mark with A.C.S. acetone.
- 0.359 mg / ml X 12 X 1 ml / 100 ml = 0.043 mg / ml
- This is the TEST solution
- Convert mg to micrograms.
- 0.043 mg/ml X 1000 micrograms / 1 milligram = 43 micrograms

Each one (1) ml of the TEST solution contains 43 micrograms of permethrin.