DILUTION OF BIOMIST 4+4 ULV FOR THE
DRAFT DOCUMENT FOR
PHEREC Technical Memorandum # 3
The following procedure yields permethrin at a dosage appropriate for
testing insecticide resistance by means of the bottle bioassay as determined by repeated
test runs at PHEREC.
- Start with the
- According to the
label one gallon of BIOMIST 4+4 ULV contains 0.3 pounds of permethrin.
pounds/gallon to grams/liter.
- 0.3 pounds/ 1
gallon X 453.6 grams/ 1 pound X 1 gallon/ 3.785 liters = 35.952 grams / liter
- Convert grams /
liter to milligrams/milliliter
- 35.952 grams /
liter X 1 gram / 1000 milligrams X 1000 milliliters / 1 liter = 35.952 milligrams /
- Dilute 1
milliliter of BIOMIST 4 + 4 ULV in 100 milliliters A.C.S. acetone.
- 35.952 milligrams
/ milliliter X 1 ml / 100 ml = 0.359 mg / ml
- This is the STOCK
The STOCK solution can be stored in a light proof sealed container
The TEST solution is prepared as follows:
- Pipette 12 ml of
the STOCK solution into a 100 ml volumetric flask.
- Fill the
volumetric flask to the 100 ml mark with A.C.S. acetone.
- 0.359 mg / ml X 12
X 1 ml / 100 ml = 0.043 mg / ml
- This is the TEST
- Convert mg to
- 0.043 mg/ml X 1000
micrograms / 1 milligram = 43 micrograms
Each one (1) ml of the TEST solution contains 43 micrograms of