**DILUTION OF DIBROM® FOR THE BOTTLE BIOASSAY**

DRAFT DOCUMENT FOR REVIEW

PHEREC Technical Memorandum # 4.

The active ingredient in Dibrom® is Naled.

1. Start with the label.

2. 1 gallon of DIBROM® contains 14.1 pounds of Naled.

3. Convert pounds per gallon to grams per milliliter.

4. 14.1 lbs/ 1 gallon X 453.6 grams/ 1 lb X 1 gallon/3785 ml = 1.7 grams
of Naled / ml of DIBROM®.

5. Take 1 ml of DIBROM® and put it in a 100 ml volumetric flask.

6. Fill to 100 ml with A.C.S. acetone; swirl to mix.

7. This is the STOCK SOLUTION.

8. Prepare the TEST SOLUTION as follows:

9. Take 0.147 ml of the STOCK SOLUTION and put it in a 100 ml volumetric flask.

10. Fill to 100 ml with A.C.S. acetone; swirl to mix.

11. This is the TEST SOLUTION.

Measure 1 ml of the TEST SOLUTION into each 250 ml Wheaton bottle for the bioassay.

This procedure will yield 25 µg of Naled in each Wheaton bottle for the bioassay.