ISRDB Cryoprotectant database for protein crystals

Number of Result: 1777. [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] [43] [44] [45] [46] [47] [48] [49] [50] [51] [52] [53] [54] [55] [56] [57] [58] [59] [60]

Protein Name	Crystallization Method	Freezing Method
pseudechetoxin	sitting-drop vapour-diffusion	Because of the extreme thinness of the crystals and the small drop volume; particular care was required in transferring the crystals for a few seconds into PFO-125/03 (perfluoropolyether) cryoprotectant oil prior to flash-freezing in a nitrogen-gas stream.
pseudecin	sitting-drop vapour-diffusion	Data collection was performed at 100K using an N2-gas stream. Crystals were soaked for 3 min in a cryosolution containing 0.2M zinc acetate; 0.1M sodium cacodylate pH 6.5; 18% PEG 8000 and 20% glycerol. Crystals were then mounted in cryoloops and flash-cooled in liquid nitrogen.
pseudo-16mer DNA	hanging-drop vapour-diffusion	the crystals were stabilized in 20% ethylene glycol; 10% PEG 4000; 0.105M NaCl; 0.055M ADA pH 6.5 for cryocooling
pseudouridine synthase RsuA	hanging-drop vapour-diffusion	A cryoprotectant solution was developed which consisted of 0.1M HEPES pH 7.5; 2%(w/v) PEG 1000; 1.7M ammonium sulfate and 30%(v/v) glycerol. A crystal was soaked in 5 micro-l of this cryoprotectant solution for 10s before being flash-cooled in liquid nitrogen. It was then mounted on the goniometer in a stream of cold nitrogen gas at 100K.
psychrophilic cellulase	hanging-drop vapour-diffusion	the crystal was flash-frozen in supercooled nitrogen gas
pteridine reductase	hanging-drop vapour-diffusion	a crystal was dipped into a cryoprotectant composed of 25%(v/v) glycerol in the reservoir solution --> mounted in a nylon loop before being placed into the Cryostream
pullulanase	hanging-drop vapour-diffusion	Prior to data collection; the crystals were treated with cryoprotectant [30%(v/v) glycerol in reservoir solution] and flash-cooled in a nitrogen-gas stream (100K).
pullulanase type I	sitting-drop vapour-diffusion	soaked for 1min in cryoprotectant solution -> flash-frozen in a nitrogen-gas stream
purine nucleoside phosphorylase	hanging-drop vapour-diffusion	crystals were always grown in the presence of the cryoprotectant glycerol -->crystal was mounted in a nylon-fibre loop and flash-frozen in liquid nitrogen
purine nucleoside phosphorylase (DeoD)	hanging-drop vapour-diffusion	A single crystal was flash-cooled in a solution consisting of 0.1M Tris-HCl pH 8.1; 20%(v/v) 2-propanol; 20%(w/v) PEG 4000; 2mM sodium dithionite and 20%(v/v) glycerol for X-ray data collection. The crystals were flash-cooled inside the glove box in liquid propane and stored outside the box in liquid nitrogen. X-ray diffraction data sets were collected under a cold nitrogen stream at about 100K.
putative LmbE-like deacetylase	hanging-drop vapour-diffusion	Crystals were transferred stepwise into a cryoprotectant buffer consisting of the reservoir solution containing up to 20%(v/v) MPD. The crystals were mounted in nylon loops and flash-cooled in liquid nitrogen.
putative UDP-N-acetyl-D-mannosamine dehydrogenase	microbatch-under-oil method	The crystals were flash-cooled in a cryoprotectant solution consisting of the precipitant solution diluted with glycerol at 20%(v/v).
putative thiamine-biosynthesis protein PH1313	oil-microbatch method	The crystals were mounted in cryoloops; covered with an emulsion mixture of Paratone-N and 10% glycerol and then flash-cooled in a nitrogen-gas stream at 100 K. X-ray diffraction data were collected from the flash-cooled crystals at 100 K
putative transposase	hanging-drop vapor-diffusion	The crystals were flash-cooled using a cryoprotectant solution consisting of 100mM Na HEPES pH 7.5; 20%(v/v) ethanol and 30%(v/v) glycerol. Crystals were soaked in 5 micro-L cryoprotectant solution for 10 s before being flash-cooled in liquid nitrogen.
pyranose 2-oxidase	hanging-drop vapour-diffusion	Prior to data collection; the crystal was mounted in a cryoloop and swept through cryoprotectant solution containing 25% glycerol; 10% PEG 400; 10%(w/v) mme PEG 2000; 0.1M MgCl2 and 0.1M sodium acetate buffer pH 4.7 and flash-frozen in liquid nitrogen.
pyridoxal kinase	hanging-drop vapour-diffusion	Single crystals were loop-mounted and placed into a cryosolution containing 27% PEG 4000; 0.17M sodium acetate trihydrate; 0.08M Tris-HCl pH 8.0; 23% glycerol for approximately 2 min.
pyrimidine nucleoside hydrolase YeiK	hanging-drop vapour-diffusion	Crystals were transferred to an artificial mother liquor containing 25% glycerol for cryoprotection and were immediately flash-cooled in a nitrogen stream at 100K.
pyrimidine nucleoside phosphorylase TTHA1771	oil-microbatch method	The crystals were flash-cooled under a nitrogen-gas stream at 100 K using an oil-based cryoprotectant comprising 90% Paratone-N and 10% glycerol by weight.
pyrogallol-phloroglucinol transhydroxylase	sitting-drop vapour diffusion in an N2/H2 atmosphere	added 25% of methylpentanediol as cryoprotectant ->flash-frozen in the nitrogen stream
pyruvate dehydrogenase (E1)	hanging-drop vapour-diffusion	a crystal was soaked in a cryoprotectant solution containing 80%(v/v) crystallization mother liquor and 20%(v/v) glycerol for approximately 1 min --> transferred to a nylon CryoLoop --> flash-frozen in liquid nitrogen
pyruvate kinase	hanging-drop vapour-diffusion	Prior to data collection; crystals were soaked in mother liquor containing 20%(v/v) glycerol which serves as a cryoprotectant. The crystals were scooped using a cryoloop and were frozen at liquid-nitrogen temperature (100K) for data collection.
pyruvate phosphate dikinase	hanging-drop vapour-diffusion	Prior to flash-cooling; the crystals were transferred into a solution of mother liquor supplemented with 19%(w/v) PEG 8000; 19%(w/v) glycerol and 25%(v/v) MPD as a cryoprotectant.
pyruvate transaminase	microbatch method	No cryogenic liquor was used and the crystals were transferred directly from the crystal drop into liquid nitrogen. Data were collected under cryocooling conditions (in a 100 K nitrogen-gas stream).
quaternary transcription-initiation complex	hanging-drop vapour-diffusion	cryostabilization: 1)removed 2ul of stabilizer solution and replaced with 2ul of cryoprotectant solution(stabilizer plus 20-30% glycerol) every hour; 2)drawn off a proportional amount of the reservoirs solution at the same time and replaced by cryoprotectant; 3)after five additions(10ul); drawn off 5ul more and added 5ul of cryoprotectant solution -->removed from the cryostabilizer solution within 1h of stabilization with thin nylon loops -->flash-cooled in a bath of liquid propane chilled to 100K or directly in liquid nitrogen
quinohaemoprotein alcohol dehydrogenase	hanging-drop vapour-diffusion	flash-frozen after soaking for 1 min in a cryopreservative consisting of 20%(v/v) glycerol; 20%(w/v) PEG 6000; 100mM MES pH5.7 --> flash-frozen in a stream of evaporating nitrogen
quinolinate phosphoribosyltransferase	hanging-drop vapour-diffusion	all crystals were transferred to a cryoprotection solution containing 40%(v/v) PEG 300; 100mM HEPES-NaOH pH 7.5 and 200mM sodium chloride.
quinoprotein glucose dehydrogenase	hanging-drop vapour-diffusion	Crystals were frozen in a cryoprotectant containing 20%(v/v) glycerol in addition to 30%(w/v) PEG 6000; 120mM NaCl; 3mM CaCl2; 100mM HEPES pH 7.5.
quinoprotein glucose dehydrogenase	hanging-drop vapour-diffusion	Crystals were cryocooled in a solution containing 20%(v/v) ethylene glycol in addition to 30%(v/v) PEG 6000; 120mM NaCl; 3mM CaCl2; 100mM CHES pH 9.2.
quinoprotein glucose dehydrogenase	hanging-drop vapour-diffusion	Crystals were flash-frozen in a solution containing 20%(v/v) glycerol or ethylene glycol in addition to 30%(w/v) PEG 6000; 120mM NaCl; 3mM CaCl2; 100mM CHES pH 9.2.
r(GGUCACAGCCC)2	hanging-drop vapour-diffusion	Crystals were soaked in successive cryosolvents containing precipitant buffer and up to 20% xylitol before freezing in a cold nitrogen stream (98K).