I'm 99+% confident that no one being cryo'd today will ever be revived. But what good does my saying that do you? @KennethHayworth might say the opposite. So you need to consider arguments, not declarations of conf. For one set of args, see my op-ed https://www.nytimes.com/2015/10/11/opinion/sunday/will-you-ever-be-able-to-upload-your-brain.html … 1/
My biggest concern is the amount of information stored intracellularly at the molecular level controlling the dynamics/plasticity of the synapses, dendrites and neurons. So long as you are unsure of how much or which molecular information you can preserve, I think p->1 that 1/
-
-
We are not really "unsure of how much or which molecular information you can preserve". Glutaraldehyde preserves proteins, their positions, and their phosphorylation states. This includes ion channels and receptors. It preserves a range of other molecules (e.g. mRNA) in matrix.
-
It covers all the components suggested to be of importance in just about the full range of existing theoretical models.
-
Maybe the relevant question is, what wouldn't it preserve?
-
Changes to protein tertiary structure. Loss of extracellular space. Loss of small ions and molecules. Fixation artifacts arising from first few seconds of living cells reacting to fix. All of this is in the literature. For example: https://cdn.elifesciences.org/articles/05793/elife-05793-v1.pdf …
-
I don't understand. Your reference compares chemical fixation to rapid freezing. Is either of these your preferred glutaraldehyde method? The paper doesn't say anything that I can see about preservation at the molecular level. ??
-
High pressure freezing (HPF) is only possible on tiny pieces of tissue (<1mm) but is considered as close to the living biology as you can study. The paper compares glutaraldehyde fixation to HPF to quantify the artifacts in glutaraldehyde fixation.
-
Aldehyde-Stabilized Cryopreservation begins with glutaraldehyde perfusion fixation so it has all of its artifacts. Follows with a slow perfusion of inert cryoprotectant to allow for long-term storage. Result is basically the same as glutaraldehyde alone but can last indefinitely
Ολοκλήρωση συζήτησης
Νέα συζήτηση -
-
-
you'll be missing something essential. That's as to your preservation method. As to how long it will take until we could take a perfectly preserved brain and make a mind out of it -- which requires the ability to reconstruct all the informative bits of the preserved brain as 2/
-
well as to know how to dynamically assemble them into the individual's working mind -- well, we will get there someday, but I think it is a very very very long time -- that it is much deeper and harder than is easy to imagine.
Ολοκλήρωση συζήτησης
Νέα συζήτηση -
Η φόρτωση φαίνεται ότι παίρνει λίγο χρόνο.
Το Twitter ενδέχεται να βρίσκεται σε υπερφόρτωση ή να αντιμετωπίζει ένα προσωρινό πρόβλημα. Προσπαθήστε ξανά ή επισκεφτείτε τη σελίδα της Κατάστασης του Twitter για περισσότερες πληροφορίες.