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The specimens are samples of blood collected from infants by saturating marked areas of a filter paper card with blood obtained by skin puncture of the heel. In recognition of the role of Dr. Robert Guthrie in newborn screening, the specimen is now called the Guthrie spot. Each state has its own collection form on which some basic demographic information as well as the blood collection spots are recorded. Many factors determine the acceptability and quality of a specimen: the timing of collection, the provision of patient and specimen information, the collection of the blood specimen, and its subsequent handling. Problems with any of these may render a specimen invalid.