Topics

    This course will look at the following primary topics through readings and other
    resources:

    oooooBiotechnology Today
    ooooo-oDNA fingerprinting
    ooooo-ogene sequencing
    ooooo-obacterial transformation, etc.

    oooooIssues in Biotechnology
    ooooo-obiotechnological processes
    ooooo
    -ogovernment legislation
    ooooo-oethical issues


    Syllabus (subject to change)
    For each date, the assignment shown is the one that is due
    on that particular day; it should be completed and ready
    at the beginning of the class session.

    This syllabus is subject to change.

    UNIT I: INTRODUCTION TO BIOTECHNOLOGY
    date - day - assignment

    09/05 - US Opening Day

    09/06 - 1 - Read and complete course handouts, purchase books (2), three ring notebook.
    00/00 - 0 - In textbook Read Preface, Laboratory safety and skim pages 3-15.

    09/07 - 2 - Read / skim p. 15- 32. Read Intro. to Biotech lab , complete pre-lab questions.

    ..............................................................................................................................................

    09/10 - 3 - x

    09/11 - 4 - Intro to Biotech. Lab Report.

    09/12 - 5 - Read Lab Fermentation/Soda, Complete Pre-lab questions. Check out
    00/00 - 0 - the following sites on fermentation (the general background and
    00/00 - 0 - information/ biochemistry of fermentation-anaerobic cellular respiration).
    00/00 - 0 - NOTE DIFFERENCES BETWEEN AEROBIC CELLULAR RESPIRATION AND FERMENTATION:

    00/00 - 0 - http://gened.emc.maricopa.edu/bio/bio181/BIOBK/BioBookGlyc.html

    00/00 - 0 - http://pc65.frontier.osrhe.edu/hs/science/bcresp.htm

    00/00 - 0 - and web sites describing the FDA'S Policy for Foods Developed by Biotechnology;
    00/00 - 0 - note especially the first biotech rDNA product/food ingredient - chymosin (rennet),
    00/00 - 0 - and the "Flavr Savr" tomato. NOTE WHAT CHYMOSIN IS USED FOR AND HOW IT
    00/00 - 0 - IS PRODUCED:

    00/00 - 0 - http://vm.cfsan.fda.gov/~lrd/biopolcy.html

    09/13 - 6 - Bring in brew pot, small tester bottle, other materials (TBA); checkout websites
    00/00 - 0 - describing homemade soda pop. NOTE DIFFERENT TYPES OF RECIPES AND WHAT
    00/00 - 0 - YOU CAN ORDER ON THE WEB:

    00/00 - 0 - http://www.leeners.com/sodapop.html

    00/00 - 0 - http://www.rootbeerworld.com
    00/00 - 0 -
    00/00 - 0 -
    http://www.floras-hideout.com/drrecipes/mxp/noalco/index.html

    09/14 - 7 - Permission slip signed. Check out the following websites on fermentation
    00/00 - 0 - products, and products of bioprocessing: microbial fermentation of glucose.

    00/00 - 0 - Sake; NOTE THE 5 ESSENTIAL INGREDIENTS AND what koji is.
    00/00 - 0 - http://www.esake.com/Sake_Knowledge/Making_Sake/making_sake.html

    00/00 - 0 - Kimchee; NOTE THE INGREDIENTS AND ORIGIN
    00/00 - 0 - http://members.tripod.com/~sgtgov/recipe1.htm
    00/00 - 0 -
    00/00 - 0 - A few kimchee recipes!
    00/00 - 0 - http://www.pcola.gulf.net/~ghancock/kimchee.html

    00/00 - 0 - Yogurt; NOTE HOW THIS IS AN EXAMPLE OF BIOTECH -- FERMENTATION!
    00/00 - 0 - http://www.uwrf.edu/biotech/workshop/activity/act1/labyo.htm

    00/00 - 0 - Cheese; BROWSE A HISTORY OF MAKING CHEESE
    00/00 - 0 - http://www.cheesemaking.com/history.html
    00/00 - 0 -
    00/00 - 0 -
    Cheese: MAKING CHEESE
    00/00 - 0 - http://www.leeners.com/cheeserecipes.html

    00/00 - 0 - Rennet: REMEMBER WHAT CHYMOSIN WAS?
    00/00 - 0 - http://arbl.cvmbs.colostate.edu/hbooks/pathphys/digestion/stomach/rennin.html

    ..............................................................................................................................................

    09/17 - 1 - Come to class with labels for soda. / Read Ward's Lab./
    00/00 - 0 - Complete Pre-Lab questions. / Look at websites on antibiotics;
    00/00 - 0 - understand the definitions and background of antibiotics:

    00/00 - 0 - Dr. Reddy's Pediatric Office on the Web
    00/00 - 0 - http://www.drreddy.com/antibx.html

    00/00 - 0 -
    00/00 - 0 -
    Bacitracin background/uses - NOTE ORIGIN AND USES OF THIS MEDICINE:

    00/00 - 0 - Hebei Shenzou Animal Medicine Co.
    00/00 - 0 - http://www.zincbacitracin.com/zincbacitracin/effect.htm

    00/00 - 0 - Hebei Shenzou Animal Medicine Co.
    00/00 - 0 - http://www.zincbacitracin.com/zincbacitracin/safety.htm

    09/18 - 3 - Rosh Hashanah NO CLASS /check soda

    09/19 - 2 - Read over procedure for Microbes at Work Lab. / Review notes.
    00/00 - 0 - Look at websites for gram staining, and note gram staining uses,
    00/00 - 0 - background, and procedure. NOTE principles and procedures section!

    00/00 - 0 - Gram Stain
    00/00 - 0 - http://www.meddean.luc.edu/lumen/DeptWebs/microbio/med/gram/gram-stn.htm

    09/20 - 4 - Set-up data charts for lab. / Work on soda lab questions (these will not be handed
    00/00 - 0 - in until soda is tasted - probably next Tuesday 09/25). / Do research for field trip:
    00/00 - 0 - Merck makes many vaccines including against Hepatitis B , infant meningitis, and
    00/00 - 0 - is working on one to combat the human papilloma virus. FIND OUT WHAT THESE
    00/00 - 0 - DISEASES INVOLVE by checking out the following links!

    00/00 - 0 - Merck's HepBinfo.com
    00/00 - 0 - http://www.hepbinfo.com

    00/00 - 0 - KidsHealth
    00/00 - 0 - http://kidshealth.org/parent/infections/bacterial_viral/meningitis_prt.htm

    00/00 - 0 - Human Papilloma Virus
    00/00 - 0 - http://www.colby.edu/health.serv/health_matters/hpv.html

    09/21 - 5 - Field trip Monday, Check out websites on Merck; note how website is set-up
    00/00 - 0 - and note bioprocessing division. The BTMC Biotechnology Manufacturing
    00/00 - 0 - Complex Facility is designed to produce vaccine and protein products
    00/00 - 0 - derived from microbial or yeast fermentation that is purified to bulk product
    00/00 - 0 - form.

    00/00 - 0 - Merck
    00/00 - 0 - http://www.merck.com

    00/00 - 0 - Our Research - Bioprocessing / R & D
    00/00 - 0 - http://www.merck.com/mrl/research/areas/bioprocess.html

    ..............................................................................................................................................

    09/24 - 6 - Come to class dressed and prepared for field trip; details to follow.

    09/25 - 7 - Bring back soda to school! Take notes on things you learned/observed from
    00/00 - 0 - field trip (to be collected/discussed) , We will continue working on soda lab
    00/00 - 0 - and microbes at work lab and use this class to wrap up loose ends from both
    00/00 - 0 - labs and to talk about the field trip.

    09/26 - 1 - Soda lab is due.

    09/27 - 3 - Yom Kippur NO CLASS

    09/28 - 2 - Microbes at work lab due. / Come to USCALC and begin major assignment, on current
    00/00 - 0 - products in the biotechnology pipeline. DUE DATE IS THURSDAY OCTOBER 4TH.

    ..............................................................................................................................................

    10/01 - 4 - Go to USCALC or work on assignment for Tuesday.

    10/02 - 5 - Read Foreword and Introduction in Genome, and Chapter 1 ("Life"). / Complete vocab and
    00/00 - 0 - questions.

    10/03 - 6 - Skim p. 75 – 93. / Define vocab words (1 – 20) on question sheet; complete the ones you are not
    00/00 - 0 - familiar with.

    10/04 - 7 - Paper due. / Read through Lab.
    00/00 - 0 - LAB - "Extraction of DNA from Different Sources".

    10/05 - x Faculty In-Service Day

    ..............................................................................................................................................

    10/08 - x Columbus Day

    10/09 - 1 - Work on Lab questions. / CURRENT EVENTS

    10/10 - 2 - HW - Read Chapter 3 ("History") in Genome; complete vocab and questions. /
    00/00 - 0 - Go to USCALC and work on "The Road to Biotechnology".

    10/11 - 3 - x

    10/12 - 4 - In USCALC work on group project "The Road to Biotechnology", due 10/17.
    00/00 - 0 - LAB - "Extraction of DNA from Different Sources" due.

    ..............................................................................................................................................

    10/15 - 5 - Carefully read "An Adventure in Dog Hair" part I and part II (you will work on these
    00/00 - 0 - in class - to be handed in on 10/16).

    10/16 - 6 - Check out VetGen... the leader in veterinary genetic disease research...
    00/00 - 0 - http://www.vetgen.com. Look at their home page, and then go to the services
    00/00 - 0 - section and then to "Revolutionary ChromaGene- coat color prediction for Labrador
    00/00 - 0 - retrievers" Note what this company markets and the genetics involved! / "Adventure in
    00/00 - 0 - Dog Hair" activity is due.

    10/17 - 7 - "Road to Biotechnology" project due. / LAB - "The Road to Biotechnology": discuss and
    00/00 - 0 - put up timeline

    10/18 - 1 - Check out the following websites:
    00/00 - 0 -
    00/00 - 0 -
    Human Genome Project Information
    00/00 - 0 - http://www.nhgri.nih.gov/HGP.
    00/00 - 0 - Note what the HGP is, when it began, the goals, and how it is managed.

    00/00 - 0 - ELSI (Ethical, Legal, and Social implications) Research Program of the
    00/00 - 0 - National Human Genome Research Institute
    00/00 - 0 - http://www.nhgri.nih.gov:80/About_NHGRI/Der/Elsi
    00/00 - 0 - Note: what its main goals are in connection with the Human Genome Project.

    ..............................................................................................................................................
    ..............................................................................................................................................


    12/17 - 5 - No assignment due. In class work on iDNA simulation

    00/00 - 0 - IDNAfication site at Southwest Biotechnology and Informatics Center
    00/00 - 0 - http://nbif.org

    12/18 - 6 - No assignment due. In class work on iDNA simulation

    12/19 - 7 - LAB. Movie: Nova - "Cracking the Code". In class work on iDNA simulation.
    00/00 - 0 - No assignment due.

    12/20 - 2 - PCR Chromosome 8 lab due. In class work on iDNA simulation/film.

    12/21 - 1 - No assignment due. In class work on iDNA simulation/film.

    01/07 - 3 - x

    01/08 - 4 - Course Evaluation due. Thank you note due. Discuss Exam.

    01/09 - 5 - Class time to work on exam alternative.

    01/10 - 6 - Class time to work on exam alternative.

    01/11 - 7 - Exam alternative due.

    .............................................................................................................................................

    T 1/22 5
    FIRST DAY SPRING SEMESTER
    In class-Review exam, Overview of Semester

    W 1/23 6
    Read “Your World” Biotechnology and You Fighting Cancer with Biotechnology
    Answer questions on handout
    In Class – discuss reading

    TH 1/24 7
    LAB
    Read article and answer questions on handout
    Scientific American Nov. 1998
    "
    Evolution and the Origins of Disease"
    http://www.sciam.com/1998/1198issue/1198nesse.html
    In class- discuss Article, and do activity Variations in Human Population-USCALC

    F 1/25 1
    CURRENT EVENTS-USCALC
    Complete Activity Variations in Human Population

    M 1/28 2
    Read Three items -Human mtDNA Lab
    Text p. 198-199, and Scientific American March 1999
    "
    What happens to sperm mitochondria?"
    http://130.94.24.217/1999/0399issue/0399scicit1.html
    In class- Finish Discussion Variations in Human Population
    Begin discussion of mt DNA

    T 1/29 3
    NO CLASS

    W 1/30 4
    Read Scientific American Jan 2001
    "
    Modern human origin morass"
    http://www.sciam.com/explorations/2001/012901human/index.html
    In class- Movie

    TH 1/31 5
    No Assignment due (work on Genome for next week)
    In class- USCALC
    Human mtDNA activity

    F 2/1 6
    Read Ancient ancestry and Mitochondrial eve articles
    In class- Finish mt DNA activity

    M 2/4 7
    LAB mt DNA
    No assignment due
    isolate hair sheath mt DNA- set-up PCR reactions (PCR over night)
    pour gels

    T 2/5 1
    CURRENT EVENTS
    In class- Discuss mt DNA activity

    W 2/6 2
    No Assignment due
    In class- Run gels, stain, analyze. Decide on samples to sequence

    TH 2/7 3
    NO CLASS

    F 2/8 4
    Read Genome Chapters Species(2) and PreHistory(13) Answer questions
    In class –Post lab Discuss Genome

    M 2/11 5
    No Assignment due
    In class –Discuss Genome, work on lab report

    T 2/12 6
    Human mt DNA lab due, Read Student Activity 27 in text
    In class- Start Unit Bioinformatics and Evolutionary Analysis of Proteins

    W 2/13 7
    LAB: Amylase and Bioinformatics Lab
    Bring in Items to test for amylase (dog or cat saliva!!)

    TH 2/14
    NO SCHOOL FACULTY INSERVICE

    F 2/15
    NO SCHOOL FACULTY INSERVICE

    M 2/18
    NO SCHOOL PRESIDENT’S DAY

    T 2/19 1
    CURRENT EVENTS
    In class- Look at results from lab Discuss Lab 28

    W 2/20 2
    Read Lab 28 Electrophoresis of Amylase Samples
    In class prep samples, run gels

    TH 2/21 3
    NO CLASS- (please come in and look at gels!)

    F 2/22 4
    Read Lab 29 Construction an Amylase Evolutionary Tree
    And Find out what a cladogram is!
    In class- discuss and start activity

    M 2/25 5
    No Assignment
    In class Finish lab 29

    T 2/26 6
    Lab 29 due
    Read lab 30 Bioinformatics
    In class- USCALC

    W 2/27 7
    No Assignment due
    LAB- Bioinformatics
    USCALC

    TH 2/28 1
    CURRENT EVENTS
    U
    SCALC- Finish lab 30/work on lab report

    F 3/1 2
    Lab 30 Report Due
    In class- Post lab/Bioinformatics

    M 3/4 3
    NO CLASS

    T 3/5 4
    (Ms. Pizzino not here- mbio f. trip) Work on Genome Chap 20 Politics

    W 3/6 5
    Read and Answer questions Chap 20 Genome Politics
    In class- discuss assignment

    TH 3/7 6
    Read Lab Somethin’ is Fishy-Protein Evolution Lab PAGE
    In class-Pre-Lab

    F 3/8 7
    INTERIM LAB- PAGE fish proteins
    Read over procedure
    In class- Prep samples, load/run samples

    M 3/11 1
    CURRENT EVENTS
    No Assignment due (work on lab questions)
    In class Observe gels, Post lab

    T 3/12 3
    NO CLASS

    W 3/13 2
    No Assignment due (work on lab report)
    In class- Post lab

    TH 3/14 4
    Lab Report Protein fingerprinting due
    In class- wrap up unit