Taurine
A compilation by Ch. M. Ruessheim
December 30, 2000
General
Taurine, one of the lesser-known amino acids, plays several important roles in the body and is essential to newborns of many species. Along with methionine, cystine and cysteine, it is a sulfur amino acid. The taurine molecule (H2N-CH2-CH2-SO2H) is small and consists of hydrogen (H), nitrogen (N), carbon (C), sulfur (S) and oxygen (O).
Most amino acids have a L- or D-configuration, which means the molecule when put into a solution will rotate light either to the left (Levo=L) or the right (Dextro=D). Taurine, like the amino acid Glycine does not polarize light and consequently does not have an L- or D-configuration.It occurs in the body as a free molecule and is never incorporated into muscle proteins. The taurine molecule is water soluble and thus doesnt easily cross the mostly fatty membranes of the bodys cell but it is present in all membranes.
For a long time, taurine was considered a nonessential nutrient for humans.
However, in recent years it has become clear that Taurine is a very important amino acid involved in a large number of metabolic processes and can become essential under certain circumstances. Taurine is important in the visual pathways, the brain and nervous system, cardiac function, and it is a conjugator of bile acids. Basically, its function is to facilitate the passage of sodium, potassium and possibly calcium and magnesium ions into and out of cells and to stabilize electrically the cell membranes. Dr. G. E. Gaull (1984) suggests that since human never develop a high level of cysteinsulfinic acid decarboxylase, an enzyme necessary for the formation of taurine from the amino acid cysteine, people are probably all somewhat dependent upon dietary taurine. Under certain conditions of high stress or in disease states the need for taurine probably increases. Another important function of taurine is detoxification.Taurine is required for efficent fat absorption & solubilization. Studies also showed that dietary taurine supplementation ameliorates experimental renal disease including models of refractory nephrotic syndrome and diabetic nephropathy. The benefinical effects of taurine are mediated by its antioxidant action. (Trachtman H. and Sturman J.A., 1996, Amino Acids, 11:1-13). Taurine may also have an important role in renal development. One study with rats showed protective effect of taurine on TNBS-induced inflammatory bowel disease. With all these discoveries and more on the horizon taurine research is accelerating rapidly.Taurine is extremely well-absorbed and a good blood level is readily obtained.
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Males in some species seem to have higher levels of the enzymes needed for taurine synthesis than do females. In monkeys, dietary taurine deficiency has a greater effect on females than on males.
Major locations in the body: In 1984, Dr. M. Shimada and his colleagues fed radioactively tagged taurine to rats. They found that the taurine went mostly to the cortex of the kidney, the liver, pituitary, thymus and adrenal glands, the eye, basal mucous membranes, salivary glands, heart and the mucous membranes lining the digestive tract. Unfortunately, they had no data on the brain. Taurine is found in high concentrations in the eye. It is the most abundant amino acid in the retina of all species studied.
Cats and Taurine
Furthermore, a number of studies have shown that immune responsiveness can be adversely affected by inappropriate taurine levels. Studies have shown, that there are changes in the host defense mechanisms in cats fed diets deficient in taurine. These host-defense mechanisms are present to protect individuals against infections with pathogenic organisms (bacteria, viruses, parasites) and against the development and spread of malignant tumors. Histological changes in the spleens of taurine-depleted cats were consistent with depletion of B-cells and T-cells and follicular center reticular cells. Lung lavage fluid from cats fed taurine-deficient diets also contained a reduced proportion of neutrophils and macrophages wich produced increased quantities of reactive oxygen intermediates associated with lower Taurine concentrations.
Taurine deficiency has also a profound adverse effect on feline pregnancy and outcome of the progeny. Such females have difficulties in maintaining their pregnancies, frequently resorbing or aborting their fetuses. Pregnancies which do reach term, frequently result in stillborn and low-birth-weight kitttens, survivors have abnormally slow growth rates and exhibit a variety of neurological symptoms. Abnormal hind-limb development and thoracic kyphosis, which appears as a dorsoventral flattening of the thoracic cavity, may happen.
The determination of a "safe" minimal dietary requirement of taurine for cats has been particularly challenging, as loss of taurine from the body is dependent on diet composition. While minimal requirements of taurine for various physiological states of cats are not known, the metabolic basis for the requirement varying with diet is at least partially understood. Expanded dry diets containing 1200mg taurine/kg DM and canned diets containing 2500 mg taurine/kg DM would appear to meet the requirements of the majority of cats. 0
Little attention has been paid to potential effects of high taurine diets, however. One study reported adverse effects in the guinea pig comprising of fatty changes in the liver accompanied by changes in the lipid content after 14 days of oral administration of taurine (Cantafora A. Mantovani A. Masella R. Mechelli M. and Alvaro D, 1986, Effect of taurine administration on liver lipids in guinea pig, Experientia 42:407).
But a study done by John A. Sturman and Jeffrey M. Messing (Department of Developmental Biochemistry, Institute for Basic Research in Developmental Disabilities Staten Island, NY) showed no adverse effect of prolonged feeding of a high taurine diet (1% DM). The diet had no effect on appetite, food consumption, weight gain or estrus cycle of the adult females. The reproductive performance, if anything, was slightly better in the females fed the high taurine diet compared to the control (0.05% and 0.2% DM). The only significant difference between cats fed either a 0.05% or a 1% fortified diet was seen in the liver lipid composition.
Interaction, Synthesis, ExcretionTaurine, as the most abundant urinary metabolite after sulfate, is formed primarily from the amino acid cysteine. Pyridoxal phosphate (active vitamin B6) is necessary for this to take place. Taurine excretion is reduced in B6 deficiency, which suggests that adequate B6 intake is necessary for the production of taurine. Some taurine may be made directly from sulfate, thus bypassing the need for cysteine. Cysteine and B6 are useful to boost taurine levels without giving taurine directly when there is concern about an irritating effect on the digestive tract. Taurine is excreted in two ways. It is readily eliminated in the urine if body taurine levels are adequate. In times of taurine depletion, however, the kidney reabsorbs taurine and does not allow it to be lost in the urine. Taurine is also excreted in the bile, where it is bound to bile acids. It probably keeps bile acids soluble, preventing gallstone formation. Interesting: When a normal nutrient balance study is done on cats consuming diets adequate in taurine, the sum of the quantity of taurine recovered in feces and urine is considerably less than the taurine ingested, even disregarding synthesis. The reason for this disparity between taurine ingested and taurine excreted must be due to microbial degradation in the gut. Anaerobic bacteria deconjugate the bile acid and degrade taurine as well as modifying the structure of the steroid moiety to produce secondary bile acids. The metabolism in the gut is therefore important in determining the taurine status of cats.
Hormonal Effects
Detoxification
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Item | Uncooked Mean |
Range | Baked Mean |
Range | Boiled Mean |
Range |
Beef muscle | 362 | 150-472 | 133 | 96-125 | 60 | 58-63 |
Beef liver | 192 | 144-270 | 141 | 68-184 | 73 | 36-95 |
Beef kidney | 225 | 180-247 | 138 | 130-144 | 76 | 68-88 |
Lamb muscle | 473 | 446-510 | 257 | 220-284 | 126 | 91-184 |
Lamb kidney | 239 | 128-440 | 154 | 81-290 | 51 | 47-55 |
Pork muscle | 496 | 394-690 | 219 | 126-390 | 118 | 91-184 |
Pork liver | 169 | 110-228 | 85 | 70-100 | 43 | 30-54 |
Chicken muscle | 337 | 300-380 | 229 | 140-310 | 82 | 71-180 |
Cod Fish | 314 | 233-396 | 294 | 260-328 | 161 | 125-198 |
Oysters | 698 | 390-1238 | 264 | 217-308 | 89 | 59-122 |
Clams | 2400 | 1450-3700 | 1017 | 587-1700 | 446 | 264-794 |
The results were either comparable or higher than those reported by these latter authors: the differences ranged from 19% and 23% higher for uncooked beef and pork, respectively to 768% higher for cooked, dark chicken meat. These differences may, in part, be a reflection of improved detection methods, differences in sample selection methods or differences in definitions of edible portions. Roe and Weston consistently found that appreciable losses of taurine occurred when meats were baked (range of losses 7-63%). When samples were boiled, the losses of taurine were even higher, ranging from 49 to 87% of the taurine found in uncooked samples. In contrast, Laidlaw et al. were generally unable to demonstrate appreciable losses of taurine from cooked meat samples by the cooking methods they used, which generally resembled baking.
High levels of taurine were observed in some seafoods as has been reported previously. However, not all sea creatures contain large amounts of taurine. Shrimp, eg. have levels of taurine similar to that found in most meats.
Very high amounts (values are always mg/100g wet
weight) were found in raw scallop (827 +/- 15 mg), raw mussel (655 +/- 72mg), raw clam
(520 +/- 97mg), raw oyster (396 +/-29mg) and raw squid (356 +/- 95mg). White fish raw
still contained 151 +/-23mg, cooked 172 +/- 54mg. In meat, the taurine content of dark
turkey and chicken tended to be substantially greater than beef, veal and pork, whereas
the three latter ones were considerably higher than light turkey or chicken. Chicken light
raw 18 +/-3mg, chicken light cooked 15mg. Chicken dark raw 169mg, broiled 199mg. Turkey
light raw 30mg, roasted 11mg, turkey dark raw 306 +/- 69 mg, roasted 299 +/-52 mg. Beef
raw 43mg, broiled 38mg. Veal raw 40mg, broiled 47mg. Pork loin raw 81 mg, roasted 57mg.
(Laidlaw Stewart A., Grosvenor M., Kopple Joel D. The Taurine Content of Common
Foodstuffs, Journal of Parenteral and Enteral Nutrition, 1990, 14, No. 2, 183-188).
Here are some more values for taurine content of meat, poultry, aquatic and other
products (adapted from Zhao Xi-he in Asia Pacific J Clin Nutr, 1994, 3, 131-134; Boren JC,
Lochmiller RL, Leslie Jr. DM in Proc Okla Acad Sci, 1996, 76, 55-65; Pasantes-Morales H,
Quesada O, Alcocer L, Sanchez-Olea R in Nutr Rep Int, 1989, 40, 793-801 and Laidlaw SA,
Grosvenor M, Kopple JD, opiter cited). Values are mg/kg except for quail serum and yogurt
which is mg/l.
Food | Taurine content | Food | Taurine content |
Conch (Strombus gigas) | 8500 | Eel | 910 |
Inkfish | 6720 | Pork meat | 1180 |
Blood Clam | 6170 | Pork heart | 2000 |
Shellfish | 3320 | Pork kidney | 1200 |
Crab | 2780 | Pork liver | 420 |
Prawn | 1430 | Chicken breast | 260 |
Sole | 2560 | Chicken leg | 3780 |
Crucial carp | 2050 | Quail muscle | 95-280* |
Silver carp | 900 | Quail serum | 0,50-0,9* |
Hairtail fish | 560 | Tuna canned | 3320 |
Yellowfish | 880 | Low-fat plain yogurt | 7.8 |
Octopus | 3900 | Shrimp | 1150 |
Cat, entire body | 2000 | Cheetah serum | 0.8-6.3 |
. | . | Cat serum | 6-14 |
* Taurine content considerably declined in tissue and serum when quails where fed a high protein diet.
Cow's milk and milk products in general don't contain a lot of taurine, eggs virtually none. Taurine content in milk varies greatly beween species and depends also on time of lactation. Rassin et al. has established an interesting overview in 1978 about various taurine concentrations in milk of diverse species:Taurine Concentration in Milk of Diverse Species |
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Species |
Concentration |
Amount of free AA |
Gerbil | 5.95 | 43.8 |
Cat | 2.87 | 71.8 |
Dog | 1.91 | 75.5 |
Rhesus Monkey | 0.56 | 33.1 |
Mouse | 0.75 | 25.4 |
Human | 0.34 | 13.0 |
Chimpanzee | 0.26 | 6.6 |
Rat | 0.15 | 9.6 |
Sheep | 0.14 | 14.2 |
Rabbit | 0.14 | 4.2 |
Cow | 0.01 | 1.8 |
Horse | 0.03 | 1.6 |
Guinea Pig | 0.56 | 3.4 |
Pasantes-Morales also researched taurine concentration in vegetables, fruits and nuts and although their contents are negligible to none here are some values for information purposes (values = nmoles/g): Walnut 15.4, Almond 17.9, Cashew 38.3, Hazelnut 46.8, Pumpkinseeds 13.5, Chick pea 18.7.
There's always the question how much taurine may be present in a whole mouse carcass since it is a natural prey of cats. So far, I was not able to find any scientific source to verify data. C.J. Puotinen claims that a typical mouse will contain 2.4 mg/g taurine or (for better comparison with the other values: 100 g would contain 240mg or 1 kg of mouse carcass would raise these values up to 2400 mg). Unfortunately, he does not indicate his source of reference. The same values have been published in Small Animal Clinical Nutrition, 4th Edition, various contributors, published 2000 by Mark Morris Institute. Well, actually they say that the taurine content of mouse carcass is 7000mg/kg but on a dry matter basis. Since mice are 60-65% water the conversion will bring it down to the values Puotinen published. Again, no source of reference. If these values are correct then - compared to Laidlows findings - dark turkey meat sure would meet the values of an equal amount of mouse carcass or even exceed it.
Recommended Reading
coming soonReferences and Abstracts
Taurine Abstracts: Part 1, Taurine & Cats: Diet, Reproduction & Development, Heart Failure