The effects of adding carotenoids from various sources to the feed of juvenile freshwater crayfish Cherax quadricarinatus, on pigmentation, growth and survival, were examined under laboratory conditions. Juvenile crayfish were fed diets enhanced with carotenoids at a level of 100 µg/g for 49 or 55 days. Carotenoid sources were: dried algal cells prepared from Dunaliella salina in which the main carotenoid is β-carotene; a synthetic carotenoid, astaxanthin (Carophyll Pink® 8%, manufactured by Hoffman-La Roche) and alfalfa meal. Crayfish receiving feeds augmented with carotenoids exhibited better body coloration than those in the control group, which were fed a diet to which no carotenoids were added. Growth and survival of the crayfish were not affected by the addition of carotenoids to their diet.