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Review Questions

  1. What genes are expressed at high-cell-density in V. cholerae?
    1. Toxin genes
    2. Biofilm genes
    3. Protease genes
    4. Attachment genes
    5. All of the above

  2. What is the order of events in V. cholerae infection?
    1. Ingestion, attachment (TcpA), bacterial cell accumulation, toxin release, detachment (proteases), escape from host.
    2. Ingestion, attachment (TcpA), toxin release, bacterial cell accumulation, detachment (proteases), escape from host.
    3. Ingestion, bacterial cell accumulation, attachment (TcpA), toxin release, detachment (proteases), escape from host.
    4. None of the above.

  3. Adding CAI-1 to a culture of V. cholerae results in:
    1. a decrease in TcpA production
    2. an increase in TcpA production
    3. no change in TcpA production

  4. What is unique about the timing of virulence gene expression in V. cholerae compared to many other pathogens?

  5. What method was used to measure activity of the CAI-1 autoinducer?



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