Why use Cas9:sgRNA libraries for functional genomic screens?
As Cas9 target specificity is determined by short 20bp sequences of the sgRNA, large scale oligo synthesis of guide sequences suggests a new ...
Introduction
In order to use the lentiCRISPR plasmid to knock out individual genes you will first need to clone in a spacer sequence to target your gene of interest (Protocol 1 below). To use the GeCKO ...
lentiCRISPRv2 (available on Addgene): Single vector with Cas9 and sgRNA on same virus, plasmid map on benchling in GenBank and FASTA formats)
lentiCas9-Blast (Addgene) & lentiGuide-Puro (Addgene): ...