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Plant Physiol Biochem. 2017 Oct;119:224-231. doi: 10.1016/j.plaphy.2017.08.017. Epub 2017 Aug 26.

Spectral phasor analysis reveals altered membrane order and function of root hair cells in Arabidopsis dry2/sqe1-5 drought hypersensitive mutant.

Author information

1
Departamento de Biología Vegetal, Laboratorio de Bioquímica, Facultad de Agronomía, Montevideo, Uruguay.
2
Departamento de Biología Vegetal, Laboratorio de Bioquímica, Facultad de Agronomía, Montevideo, Uruguay. Electronic address: msotelo@fagro.edu.uy.
3
Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay.
4
Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain.
5
Departamento de Fisiopatología, Hospital de Clínicas, Universidad de la Republica, Montevideo-Uruguay, Analytical Biochemistry and Proteomics Unit, Institut Pasteur of Montevideo, Uruguay.

Abstract

Biological membranes allow the regulation of numerous cellular processes, which are affected when unfavorable environmental factors are perceived. Lipids and proteins are the principal components of biological membranes. Each lipid has unique biophysical properties, and, therefore the lipid composition of the membrane is critical to maintaining the bilayer structure and functionality. Membrane composition and integrity are becoming the focus of studies aiming to understand how plants adapt to its environment. In this study, using a combination of di-4-ANEPPDHQ fluorescence and spectral phasor analysis, we report that the drought hypersensitive/squalene epoxidase (dry2/sqe1-5) mutant with reduced major sterols such as sitosterol and stigmasterol in roots presented higher membrane fluidity than the wild type. Moreover, analysis of endomembrane dynamics showed that vesicle formation was affected in dry2/sqe1-5. Further analysis of proteins associated with sterol rich micro domains showed that dry2/sqe1-5 presented micro domains function altered.

KEYWORDS:

Arabidopsis thaliana; Drought hypersensibility; Membranes fluidity; SQE1; Spectral phasor

PMID:
28910707
DOI:
10.1016/j.plaphy.2017.08.017
[Indexed for MEDLINE]

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