Etched Carbon-Fiber Electrodes as Amperometric Detectors of Catecholamine Secretion From Isolated Biological Cells
- PMID: 1952084
- DOI: 10.1021/ac00015a017
Etched Carbon-Fiber Electrodes as Amperometric Detectors of Catecholamine Secretion From Isolated Biological Cells
Abstract
Voltammetric electrodes with micrometer dimensions have been fabricated from carbon fibers etched to a conical shape and insulated with poly(oxyphenylene) following literature procedures. The resultant electrode has a tip radius in the micron range. The response of this electrode is compared to a carbon-fiber electrode prepared by sealing a carbon-fiber electrode in a glass pipet (electrode radius greater than 3.5 microns). While the etched electrodes did not follow electrochemical theory as well as the glass-encased electrode, the etched electrode was found to be suitable for the amperometric measurement of the secretion of catecholamines from isolated bovine adrenal cells. Comparable results are only obtained when the two different electrodes are placed 1 micron from the cell surface. When the etched electrode is placed further away, less secretion is observed, because of diffusion and accompanying dilution.
Similar articles
-
Temporal Characteristics of Quantal Secretion of Catecholamines From Adrenal Medullary CellsJA Jankowski et al. J Biol Chem 268 (20), 14694-700. PMID 8325848.Exocytotic release of vesicular catecholamine from individual bovine adrenal medullary cells was detected with carbon fiber microelectrodes. Release was elicited from cel …
-
On-chip Amperometric Measurement of Quantal Catecholamine Release Using Transparent Indium Tin Oxide ElectrodesX Sun et al. Anal Chem 78 (8), 2521-5. PMID 16615759.Carbon-fiber amperometry has been extensively used to monitor the time course of catecholamine release from cells as individual secretory granules discharge their content …
-
Amperometric Detection of Quantal Catecholamine Secretion From Individual Cells on Micromachined Silicon ChipsP Chen et al. Anal Chem 75 (3), 518-24. PMID 12585478.We have fabricated electrochemical electrodes in picolitersized wells for measuring catecholamine release from individual cells with millisecond resolution. Each well-ele …
-
Simultaneous Amperometric Measurement of Ascorbate and Catecholamine Secretion From Individual Bovine Adrenal Medullary CellsPS Cahill et al. Anal Chem 67 (15), 2599-605. PMID 8849026.Secretion of ascorbate and catecholamines from single bovine adrenal medullary cells has been detected with amperometry at carbon-fiber microelectrodes. Two carbon-fiber …
-
Improved Surface-Patterned Platinum Microelectrodes for the Study of Exocytotic EventsK Berberian et al. Anal Chem 81 (21), 8734-40. PMID 19780579.Surface-patterned platinum microelectrodes insulated with 300 nm thick fused silica were fabricated using contact photolithography. These electrodes exhibit low noise and …
Cited by 22 PubMed Central articles
-
Recent Advances in Fast-Scan Cyclic VoltammetryP Puthongkham et al. Analyst 145 (4), 1087-1102. PMID 31922162.Fast-scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes (CFMEs) is a versatile electrochemical technique to probe neurochemical dynamics in vivo. Progress in …
-
Analysis of Carbon-Based Microelectrodes for Neurochemical SensingFS Manciu et al. Materials (Basel) 12 (19). PMID 31569398.The comprehensive microscopic, spectroscopic, and in vitro voltammetric analysis presented in this work, which builds on the well-studied properties of carbon-based mater …
-
Review: New Insights Into Optimizing Chemical and 3D Surface Structures of Carbon Electrodes for Neurotransmitter DetectionQ Cao et al. Anal Methods 11 (3), 247-261. PMID 30740148.The carbon-fiber microelectrode has been used for decades as a neurotransmitter sensor. Recently, new strategies have been developed for making carbon electrodes, includi …
-
Regional Differences in Dopamine Release in the R6/2 Mouse Caudate PutamenSV Kaplan et al. Electroanalysis 30 (6), 1066-1072. PMID 29955208.Huntington's disease (HD) is a fatal neurodegenerative disorder that is characterized by degeneration of the striatum. Here, fast-scan cyclic voltammetry at carbon-fiber …
-
Spatial and Activity-Dependent Catecholamine Release in Rat Adrenal Medulla Under Native Neuronal StimulationK Wolf et al. Physiol Rep 4 (17). PMID 27597763.Neuroendocrine chromaffin cells of the adrenal medulla in rat receive excitatory synaptic input through anterior and posterior divisions of the sympathetic splanchnic ner …
Publication types
MeSH terms
Substances
Grant support
LinkOut - more resources
Other Literature Sources