Trypanosoma brucei spp. development in the tsetse fly: characterization of the post-mesocyclic stages in the foregut and proboscis
- PMID: 10363280
- DOI: 10.1017/s0031182099004217
Trypanosoma brucei spp. development in the tsetse fly: characterization of the post-mesocyclic stages in the foregut and proboscis
Abstract
Post-mesocyclic development of Trypanosoma brucei in the tsetse fly in its migration from midgut to salivary glands, was revisited by sequential microdissection, morphometry and DNA-cytofluorometry. This development started by day 6 after the infective feed, with passage of mesocyclic midgut trypomastigotes through proventriculus and upward migration along foregut and proboscis to the salivary gland ducts. Kinetics of salivary gland infection showed that colonization of the salivary glands by epimastigotes occurred only during the time-limited presence of this developmental phase in the foregut and proboscis. Post-mesocyclic trypanosomes in the foregut and proboscis were pleomorphic, with 4 morphological stages in various constant proportions and present all through from proventriculus up to the salivary gland ducts: 67% long trypomastigotes, 27% long epimastigotes, 4% long epimastigotes undergoing asymmetric cell division and 2% short epimastigotes. Measurements of DNA content demonstrated a predominant tetraploidy for 67% of these trypanosomes, the remainder consisting of the homogeneous diploid short epimastigotes and some long epimastigotes. According to the experimental data, the following sequence of trypanosome differentiation in the foregut and proboscis is proposed as the most obvious hypothesis. Incoming mesocyclic trypomastigotes (2N) from the ectoperitrophic anterior midgut start to replicate DNA to a 4N level, are arrested at this point, and differentiate into the long epimastigote (4N) which give rise, by an asymmetric cell division, to 2 unequal, diploid daughter cells: a long, probably dead-end long epimastigote and a short epimastigote. The latter is responsible for the epimastigote colonization of the salivary glands if launched at the vicinity of the gland epithelium by the asymmetric dividing epimastigote.
Similar articles
-
The life cycle of Trypanosoma (Nannomonas) congolense in the tsetse fly.Parasit Vectors. 2012 Jun 27;5:109. doi: 10.1186/1756-3305-5-109. Parasit Vectors. 2012. PMID: 22676292 Free PMC article.
-
Shape-shifting trypanosomes: Flagellar shortening followed by asymmetric division in Trypanosoma congolense from the tsetse proventriculus.PLoS Pathog. 2018 May 17;14(5):e1007043. doi: 10.1371/journal.ppat.1007043. eCollection 2018 May. PLoS Pathog. 2018. PMID: 29772025 Free PMC article.
-
Tsetse fly tolerance to T. brucei infection: transcriptome analysis of trypanosome-associated changes in the tsetse fly salivary gland.BMC Genomics. 2016 Nov 25;17(1):971. doi: 10.1186/s12864-016-3283-0. BMC Genomics. 2016. PMID: 27884110 Free PMC article.
-
Flying tryps: survival and maturation of trypanosomes in tsetse flies.Trends Parasitol. 2013 Apr;29(4):188-96. doi: 10.1016/j.pt.2013.02.003. Epub 2013 Mar 16. Trends Parasitol. 2013. PMID: 23507033 Review.
-
Antigenic variation during the developmental cycle of Trypanosoma brucei.J Protozool. 1984 Feb;31(1):41-7. J Protozool. 1984. PMID: 6204043 Review.
Cited by
-
The Trypanosoma brucei MISP family of invariant proteins is co-expressed with BARP as triple helical bundle structures on the surface of salivary gland forms, but is dispensable for parasite development within the tsetse vector.PLoS Pathog. 2023 Mar 30;19(3):e1011269. doi: 10.1371/journal.ppat.1011269. eCollection 2023 Mar. PLoS Pathog. 2023. PMID: 36996244 Free PMC article.
-
Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment.Microorganisms. 2022 May 31;10(6):1141. doi: 10.3390/microorganisms10061141. Microorganisms. 2022. PMID: 35744659 Free PMC article.
-
VEX1 Influences mVSG Expression During the Transition to Mammalian Infectivity in Trypanosoma brucei.Front Cell Dev Biol. 2022 Apr 5;10:851475. doi: 10.3389/fcell.2022.851475. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 35450294 Free PMC article.
-
Targeting the tsetse-trypanosome interplay using genetically engineered Sodalis glossinidius.PLoS Pathog. 2022 Mar 10;18(3):e1010376. doi: 10.1371/journal.ppat.1010376. eCollection 2022 Mar. PLoS Pathog. 2022. PMID: 35271685 Free PMC article.
-
Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages.PLoS Pathog. 2022 Mar 7;18(3):e1010346. doi: 10.1371/journal.ppat.1010346. eCollection 2022 Mar. PLoS Pathog. 2022. PMID: 35255094 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Miscellaneous