UniProtKB - Q12874 (SF3A3_HUMAN)
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Protein
Splicing factor 3A subunit 3
Gene
SF3A3
Organism
Homo sapiens (Human)
Status
Functioni
Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 406 – 437 | Matrin-typePROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- RNA binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- mRNA 3'-splice site recognition Source: HGNC
- mRNA processing Source: HGNC
- mRNA splicing, via spliceosome Source: Reactome
- RNA splicing Source: ProtInc
- RNA splicing, via transesterification reactions Source: UniProtKB
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-HSA-72163. mRNA Splicing - Major Pathway. |
Names & Taxonomyi
Protein namesi | Recommended name: Splicing factor 3A subunit 3Alternative name(s): SF3a60 Spliceosome-associated protein 61 Short name: SAP 61 |
Gene namesi | Name:SF3A3 Synonyms:SAP61 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10767. SF3A3. |
Subcellular locationi
GO - Cellular componenti
- catalytic step 2 spliceosome Source: UniProtKB
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- spliceosomal complex Source: HGNC
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 10946. |
OpenTargetsi | ENSG00000183431. |
PharmGKBi | PA35685. |
Polymorphism and mutation databases
BioMutai | SF3A3. |
DMDMi | 17380310. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174318 | 1 – 501 | Splicing factor 3A subunit 3Add BLAST | 501 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources1 Publication | 1 | |
Modified residuei | 54 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 121 | PhosphoserineCombined sources | 1 | |
Modified residuei | 295 | PhosphoserineCombined sources | 1 | |
Modified residuei | 299 | PhosphoserineCombined sources | 1 | |
Modified residuei | 365 | PhosphoserineCombined sources | 1 | |
Modified residuei | 367 | PhosphoserineCombined sources | 1 | |
Modified residuei | 369 | PhosphoserineCombined sources | 1 | |
Modified residuei | 475 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q12874. |
MaxQBi | Q12874. |
PaxDbi | Q12874. |
PeptideAtlasi | Q12874. |
PRIDEi | Q12874. |
PTM databases
iPTMneti | Q12874. |
PhosphoSitePlusi | Q12874. |
SwissPalmi | Q12874. |
Expressioni
Tissue specificityi
Ubiquitous.
Gene expression databases
Bgeei | ENSG00000183431. |
CleanExi | HS_SF3A3. |
Genevisiblei | Q12874. HS. |
Organism-specific databases
HPAi | HPA032054. HPA032055. |
Interactioni
Subunit structurei
Identified in the spliceosome C complex. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A1, through its N-terminal region.2 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 116146. 123 interactors. |
DIPi | DIP-882N. |
IntActi | Q12874. 26 interactors. |
MINTi | MINT-1467744. |
STRINGi | 9606.ENSP00000362110. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 80 – 96 | Combined sources | 17 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DT7 | NMR | - | A | 71-107 | [»] | |
ProteinModelPortali | Q12874. | |||||
SMRi | Q12874. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12874. |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 354 – 362 | Poly-Glu | 9 |
Sequence similaritiesi
Belongs to the SF3A3 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 406 – 437 | Matrin-typePROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2636. Eukaryota. COG5188. LUCA. |
GeneTreei | ENSGT00530000063402. |
HOGENOMi | HOG000160935. |
HOVERGENi | HBG054452. |
InParanoidi | Q12874. |
KOi | K12827. |
OMAi | EFPCEIC. |
OrthoDBi | EOG091G059G. |
PhylomeDBi | Q12874. |
TreeFami | TF315227. |
Family and domain databases
InterProi | View protein in InterPro IPR024598. DUF3449. IPR000690. Matrin/U1-C_Znf_C2H2. IPR031776. SF3A3. IPR031774. SF3A3_dom. IPR021966. SF3a60_bindingd. |
PANTHERi | PTHR12786:SF4. PTHR12786:SF4. 1 hit. |
Pfami | View protein in Pfam PF11931. DUF3449. 1 hit. PF16837. SF3A3. 1 hit. PF12108. SF3a60_bindingd. 1 hit. |
PROSITEi | View protein in PROSITE PS50171. ZF_MATRIN. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q12874-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY
60 70 80 90 100
MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLKQ IKEFHRKHPN
110 120 130 140 150
EICVPMSVEF EELLKARENP SEEAQNLVEF TDEEGYGRYL DLHDCYLKYI
160 170 180 190 200
NLKASEKLDY ITYLSIFDQL FDIPKERKNA EYKRYLEMLL EYLQDYTDRV
210 220 230 240 250
KPLQDQNELF GKIQAEFEKK WENGTFPGWP KETSSALTHA GAHLDLSAFS
260 270 280 290 300
SWEELASLGL DRLKSALLAL GLKCGGTLEE RAQRLFSTKG KSLESLDTSL
310 320 330 340 350
FAKNPKSKGT KRDTERNKDI AFLEAQIYEY VEILGEQRHL THENVQRKQA
360 370 380 390 400
RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK PIPYWLYKLH
410 420 430 440 450
GLNINYNCEI CGNYTYRGPK AFQRHFAEWR HAHGMRCLGI PNTAHFANVT
460 470 480 490 500
QIEDAVSLWA KLKLQKASER WQPDTEEEYE DSSGNVVNKK TYEDLKRQGL
L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 176 | E → G in CAA57388 (PubMed:7816610).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08815 mRNA. Translation: AAA19625.1. X81789 mRNA. Translation: CAA57388.1. AL603790 Genomic DNA. Translation: CAH69930.1. CH471059 Genomic DNA. Translation: EAX07304.1. CH471059 Genomic DNA. Translation: EAX07305.1. BC002395 mRNA. Translation: AAH02395.1. BC011523 mRNA. Translation: AAH11523.1. |
CCDSi | CCDS428.1. |
PIRi | A55749. |
RefSeqi | NP_006793.1. NM_006802.3. |
UniGenei | Hs.77897. |
Genome annotation databases
Ensembli | ENST00000373019; ENSP00000362110; ENSG00000183431. |
GeneIDi | 10946. |
KEGGi | hsa:10946. |
UCSCi | uc001cci.4. human. |
Similar proteinsi
Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:100% | UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry. |
90% | UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence). |
50% | UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster. |
Entry informationi
Entry namei | SF3A3_HUMAN | |
Accessioni | Q12874Primary (citable) accession number: Q12874 Secondary accession number(s): D3DPT5, Q15460, Q5VT87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | June 7, 2017 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families